LOCUS       sequence-88851-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 505 / 569, 
            Emission: 516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: wtEos-ER-4
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        42..93
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     44..64
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             185..859
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="SAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKE
                     GGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIARN
                     DITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDITMALLLE
                     GNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLSDN
                     ARR"
     misc_feature    875..931
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 tcaacgggac ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
       61 cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtca gatccgctag
      121 catgctgcta tccgtgccgt tgctgctcgg cctcctcggc ctggccgtcg ccgaccggtt
      181 cgagagtgcg attaagccag acatgaagat caacctccgt atggaaggca acgtaaacgg
      241 gcaccacttt gtgatcgacg gagatggtac aggcaagcct tttgagggaa aacagagtat
      301 ggatcttgaa gtcaaagagg gcggacctct gccttttgcc tttgatatcc tgaccactgc
      361 attccattac ggcaacaggg tattcgccga atatccagac cacatacaag actattttaa
      421 gcagtcgttt cctaaggggt attcgtggga acgaagcttg actttcgaag acgggggcat
      481 ttgcattgcc agaaacgaca taacaatgga aggggacact ttctataata aagttcgatt
      541 tcacggtgta aactttcccg ccaatggtcc agttatgcag aagaagacgc tgaaatggga
      601 gccctccact gagaaaatgt atgtgcgtga tggagtgctg acgggtgata ttaccatggc
      661 tttgttgctt gaaggaaatg cccattaccg atgtgacttc agaactactt acaaagctaa
      721 ggagaagggt gtcaagttac caggctacca ctttgtggac cactgcattg agattttaag
      781 ccatgacaaa gattacaaca aggttaagct gtatgagcat gctgttgctc attctggatt
      841 gtccgacaat gccagacgaa aggacgaggt ctaaagatct cgagctcaag cttcgaattc
      901 tgcagtcgac ggtaccgcgg gcccgggatc caccggatct agataactga tcataatcag
      961 ccataccaca tttgtagagg ttttacttgc ttta
//
