LOCUS       sequence-88871-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane, Excitation: 490 / 553, Emission: 507 / 573.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Dendra2-Farnesyl-5
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        9..60
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     11..31
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             105..794
                     /codon_start=1
                     /product="monomeric photoswitchable green-to-red 
                     fluorescent protein from Dendronephthya"
                     /label=Dendra2
                     /note="mammalian codon-optimized"
                     /translation="MNTPGINLIKEDMRVKVHMEGNVNGHAFVIEGEGKGKPYEGTQTA
                     NLTVKEGAPLPFSYDILTTAVHYGNRVFTKYPEDIPDYFKQSFPEGYSWERTMTFEDKG
                     ICTIRSDISLEGDCFFQNVRFKGTNFPPNGPVMQKKTLKWEPSTEKLHVRDGLLVGNIN
                     MALLLEGGGHYLCDFKTTYKAKKVVQLPDAHFVDHRIEILGNDSDYNKVKLYEHAVARY
                     SPLPSQVW"
ORIGIN
        1 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct
       61 ggtttagtga accgtcagat ccgctagcgc taccggtcgc caccatgaac accccgggaa
      121 ttaacctgat caaggaggac atgcgcgtga aggtgcacat ggagggcaac gtgaacggcc
      181 acgccttcgt gatcgagggc gagggcaagg gcaagcccta cgagggcacc cagaccgcca
      241 acctgaccgt gaaggagggc gcccccctgc ccttcagcta cgacatcctg accaccgccg
      301 tgcactacgg caaccgggtg ttcaccaagt accccgagga catccccgac tacttcaagc
      361 agagcttccc cgagggctac agctgggagc gcaccatgac cttcgaggac aagggcatct
      421 gcaccatccg cagcgacatc agcctggagg gcgactgctt cttccagaac gtgcgcttca
      481 agggcaccaa cttccccccc aacggccccg tgatgcagaa gaagaccctg aagtgggagc
      541 ccagcaccga gaagctgcac gtgcgcgacg gcctgctggt gggcaacatc aacatggccc
      601 tgctgctgga gggcggcggc cactacctgt gcgacttcaa gaccacctac aaggccaaga
      661 aggtggtgca gctgcccgac gcccacttcg tggaccaccg catcgagatc ctgggcaacg
      721 acagcgacta caacaaggtg aagctgtacg agcacgccgt ggcccgctac agccccctgc
      781 ccagccaggt gtggtccgga ctcagatcta agctgaaccc tcctgatgag agtggccccg
      841 gctgcatgag ctgcaagtgt gtgctctcct gagaattctg cagtcgacgg taccgcgggc
      901 ccgggatcca ccggatctag ataactgatc ataatcagcc ataccacatt tgtagaggtt
      961 ttacttgctt taaaaaacct ccacacctcc ccct
//
