LOCUS       sequence-88872-e        1775 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nuclear - Nucleolus, Excitation: 490 / 553, Emission: 
            507 / 573.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1775)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Dendra2-Fibrillarin-11
FEATURES             Location/Qualifiers
     source          1..1775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             45..734
                     /codon_start=1
                     /product="monomeric photoswitchable green-to-red 
                     fluorescent protein from Dendronephthya"
                     /label=Dendra2
                     /note="mammalian codon-optimized"
                     /translation="MNTPGINLIKEDMRVKVHMEGNVNGHAFVIEGEGKGKPYEGTQTA
                     NLTVKEGAPLPFSYDILTTAVHYGNRVFTKYPEDIPDYFKQSFPEGYSWERTMTFEDKG
                     ICTIRSDISLEGDCFFQNVRFKGTNFPPNGPVMQKKTLKWEPSTEKLHVRDGLLVGNIN
                     MALLLEGGGHYLCDFKTTYKAKKVVQLPDAHFVDHRIEILGNDSDYNKVKLYEHAVARY
                     SPLPSQVW"
     CDS             complement(1542..1553)
                     /codon_start=1
                     /gene="cspA"
                     /product="translation enhancing element for E. coli (Qing 
                     et al., 2004)"
                     /label=TEE
                     /translation="NHKV"
ORIGIN
        1 ggtttagtga accgtcagat ccgctagcgc taccggtcgc caccatgaac accccgggaa
       61 ttaacctgat caaggaggac atgcgcgtga aggtgcacat ggagggcaac gtgaacggcc
      121 acgccttcgt gatcgagggc gagggcaagg gcaagcccta cgagggcacc cagaccgcca
      181 acctgaccgt gaaggagggc gcccccctgc ccttcagcta cgacatcctg accaccgccg
      241 tgcactacgg caaccgggtg ttcaccaagt accccgagga catccccgac tacttcaagc
      301 agagcttccc cgagggctac agctgggagc gcaccatgac cttcgaggac aagggcatct
      361 gcaccatccg cagcgacatc agcctggagg gcgactgctt cttccagaac gtgcgcttca
      421 agggcaccaa cttccccccc aacggccccg tgatgcagaa gaagaccctg aagtgggagc
      481 ccagcaccga gaagctgcac gtgcgcgacg gcctgctggt gggcaacatc aacatggccc
      541 tgctgctgga gggcggcggc cactacctgt gcgacttcaa gaccacctac aaggccaaga
      601 aggtggtgca gctgcccgac gcccacttcg tggaccaccg catcgagatc ctgggcaacg
      661 acagcgacta caacaaggtg aagctgtacg agcacgccgt ggcccgctac agccccctgc
      721 ccagccaggt gtggtccggc gacagcggcg tgtacaagac tcgagccatg aagccaggat
      781 tcagtccccg tgggggtggc tttggcggcc gagggggctt tggtgaccgt ggtggtcgtg
      841 gaggccgagg gggctttggc gggggccgag gccaaggcgg aggctttaga ggtcgtggac
      901 gaggaggagg tggaggcggc ggcggcggtg gaggaggagg aagaggtggt ggaggcttcc
      961 attttggtgg caaccggggt cgtggtcggg gaggaaaaag aggaaaccag tcggggaaga
     1021 atgtgatggt ggagccgcat cggcatgagg gtgtcttcat ttgtcgggga aaggaagatg
     1081 cactggtcac caagaacctg gtccctgggg aatcagttta tggagagaag agagtctcga
     1141 tttcggaagg agatgacaaa attgagtacc gagcctggaa ccccttccgc tccaagctag
     1201 cagcagcaat cctgggtggt gtggaccaga tccacatcaa accgggggct aaggttctct
     1261 acctcggggc tgcctcgggc accacggtct cccatgtctc tgacatcgtt ggtccggatg
     1321 gtctagtcta tgcagtcgag ttctcccacc gctctggccg tgacctcatt aacttggcca
     1381 agaagaggac caacatcatt cctgtgatcg aggatgctcg acacccacac aaataccgca
     1441 tgctcatcgc aatggtggat gtgatctttg ctgatgtggc ccagccagac cagacccgga
     1501 ttgtggccct gaatgcccac accttcctgc gtaatggagg acactttgtg atttccatta
     1561 aggccaactg cattgactcc acagcctcag ccgaggccgt gtttgcctcc gaagtgaaaa
     1621 agatgcaaca ggagaacatg aagccgcagg agcagttgac ccttgagcca tatgaaagag
     1681 accatgccgt ggtcgtggga gtgtacaggc caccccccaa ggtgaagaac tgaggatcca
     1741 ccggatctag ataactgatc ataatcagcc atacc
//
