LOCUS       sequence-88913-e        2729 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Microtubules, Excitation: 458 / 475, Emission: 458 / 
            517.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2729)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Phamret-MAP4-C-10
FEATURES             Location/Qualifiers
     source          1..2729
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(34..36,37..39,40..552)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIE"
ORIGIN
        1 acgtcagatc cgctagcgct accggtcgcc accatggtga gcaagggcga ggagctgttc
       61 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caggttcagc
      121 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
      181 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctggggcgtg
      241 cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
      301 cccgaaggct acgtccagga gcgtaccatc ttcttcaagg acgacggcaa ctacaagacc
      361 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
      421 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta catcagccac
      481 aacgtctata tcaccgccga caagcagaag aacggcatca aggcccactt caagatccgc
      541 cacaacatcg aggacggcgg cgtgcagctc gccgaccact accagcagaa cacccccatc
      601 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc caagctgagc
      661 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggt
      721 accaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
      781 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
      841 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
      901 accaccttca gctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
      961 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
     1021 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     1081 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     1141 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc
     1201 aaggccaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
     1261 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     1321 agccaccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     1381 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtccggactc
     1441 agatctggtg gctccgcagg atcccggcaa gaagaagcaa aggctgctgt aggtgtgact
     1501 ggaaatgaca tcactacccc gccaaacaag gagccaccac caagcccaga aaagaaagca
     1561 aagcctttgg ccaccactca acctgcaaag acttcaacat cgaaagccaa aacacagccc
     1621 acttctctcc ctaagcaacc agctcccacc acctctggtg ggttgaataa aaaacccatg
     1681 agcctcgcct caggctcagt gccagctgcc ccacacaaac gccctgctgc tgccactgct
     1741 actgccaggc cttccaccct acctgccaga gacgtgaagc caaagccaat tacagaagct
     1801 aaggttgccg aaaagcggac ctctccatcc aagccttcat ctgccccagc cctcaaacct
     1861 ggacctaaaa ccaccccaac cgtttcaaaa gccacatctc cctcaactct tgtttccact
     1921 ggaccaagta gtagaagtcc agctacaact ctgcctaaga ggccaaccag catcaagact
     1981 gaggggaaac ctgctgatgt caaaaggatg actgctaagt ctgcctcagc tgacttgagt
     2041 cgctcaaaga ccacctctgc cagttctgtg aagagaaaca ccactcccac tggggcagca
     2101 cccccagcag ggatgacttc cactcgagtc aagcccatgt ctgcacctag ccgctcttct
     2161 ggggctcttt ctgtggacaa gaagcccact tccactaagc ctagctcctc tgctcccagg
     2221 gtgagccgcc tggccacaac tgtttctgcc cctgacctga agagtgttcg ctccaaggtc
     2281 ggctctacag aaaacatcaa acaccagcct ggaggaggcc gggccaaggt agagaaaaaa
     2341 acagaggcag ctaccacagc tgggaagcct gaacctaatg cagtcactaa agcagccggc
     2401 tccattgcga gtgcacagaa accgcctgct gggaaagtcc agatagtatc caaaaaagtg
     2461 agctacagtc atattcaatc caagtgtgtt tccaaggaca atattaagca tgtccctgga
     2521 tgtggcaatg ttcagattca gaacaagaaa gtggacatat ccaaggtctc ctccaagtgt
     2581 gggtccaaag ctaatatcaa gcacaagcct ggtggaggag atgtcaagat tgaaagtcag
     2641 aagttgaact tcaaggagaa ggcccaagcc aaagtgggat agggatccac cggatctaga
     2701 taactgatca taatcagcca taccacatt
//
