LOCUS       sequence-89009-e        3124 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Tight Junctions, Excitation: 551, Emission: 565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3124)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 3124)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKO2-Beta-Catenin-20
FEATURES             Location/Qualifiers
     source          1..3124
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             32..685
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
     regulatory      740..749
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             791..826
                     /codon_start=1
                     /product="nanobody epitope tag corresponding to amino acids
                     16-27 of human beta-catenin (Traenkle et al., 2015)"
                     /label=BC2 tag
                     /translation="PDRKAAVSHWQQ"
ORIGIN
        1 gtcagatccg ctagcgctac cggtcgccac catggtgagt gtgattaaac cagagatgaa
       61 gatgaggtac tacatggacg gctccgtcaa tgggcatgag ttcacaattg aaggtgaagg
      121 cacaggcaga ccttacgagg gacatcaaga gatgacacta cgcgtcacaa tggccgaggg
      181 cgggccaatg cctttcgcgt ttgacttagt gtcacacgtg ttctgttacg gccacagagt
      241 atttactaaa tatccagaag agataccaga ctatttcaaa caagcatttc ctgaaggcct
      301 gtcatgggaa aggtcgttgg agttcgaaga tggtgggtcc gcttcagtca gtgcgcatat
      361 aagccttaga ggaaacacct tctaccacaa atccaaattt actggggtta actttcctgc
      421 cgatggtcct atcatgcaaa accaaagtgt tgattgggag ccatcaaccg agaaaattac
      481 tgccagcgac ggagttctga agggtgatgt tacgatgtac ctaaaacttg aaggaggcgg
      541 caatcacaaa tgccaaatga agactactta caaggcggca aaagagattc ttgaaatgcc
      601 aggagaccat tacatcggcc atcgcctcgt caggaaaacc gaaggcaaca ttactgagca
      661 ggtagaagat gcagtagctc atagctccgg actcagatct cgagctcaag cttcgaattc
      721 tgcagtcgac ggtaccgcgg ccaccatggc tactcaagct gacctgatgg agttggacat
      781 ggccatggag ccggacagaa aagctgctgt cagccactgg cagcagcagt cttacttgga
      841 ttctggaatc cattctggtg ccaccaccac agctccttcc ctgagtggca agggcaaccc
      901 tgaggaagaa gatgttgaca cctcccaagt cctttatgaa tgggagcaag gcttttccca
      961 gtccttcacg caagagcaag tagctgatat tgacgggcag tatgcaatga ctagggctca
     1021 gagggtccga gctgccatgt tccctgagac gctagatgag ggcatgcaga tcccatccac
     1081 gcagtttgac gctgctcatc ccactaatgt ccagcgcttg gctgaaccat cacagatgtt
     1141 gaaacatgca gttgtcaatt tgattaacta tcaggatgac gcggaacttg ccacacgtgc
     1201 aattcctgag ctgacaaaac tgctaaacga tgaggaccag gtggtagtta ataaagctgc
     1261 tgttatggtc catcagcttt ccaaaaagga agcttccaga catgccatca tgcgctcccc
     1321 tcagatggtg tctgccattg tacgcaccat gcagaataca aatgatgtag agacagctcg
     1381 ttgtactgct gggactctgc acaacctttc tcaccaccgc gagggcttgc tggccatctt
     1441 taagtctggt ggcatcccag cgctggtgaa aatgcttggg tcaccagtgg attctgtact
     1501 gttctacgcc atcacgacac tgcataatct cctgctccat caggaaggag ctaaaatggc
     1561 agtgcgccta gctggtggac tgcagaaaat ggttgctttg ctcaacaaaa caaacgtgaa
     1621 attcttggct attacaacag actgccttca gatcttagct tatggcaatc aagagagcaa
     1681 gctcatcatt ctggccagtg gtggacccca agccttagta aacataatga ggacctacac
     1741 ttatgagaag cttctgtgga ccacaagcag agtgctgaaa gtgctgtctg tctgctctag
     1801 caacaagccg gccattgtag aagctggtgg gatgcaggca ctggggcttc atctgacaga
     1861 cccaagtcag cgacttgttc aaaactgtct ttggactctc agaaaccttt cagatgcagc
     1921 gactaagcag gaagggatgg aaggcctcct tgggactcta gtgcagcttc tgggttccga
     1981 tgatataaat gtggtcacct gtgcagctgg aattctctct aacctcactt gcaataatta
     2041 caaaaacaag atgatggtgt gccaagtggg tggcatagag gctcttgtac gcaccgtcct
     2101 tcgtgctggt gacagggaag acatcactga gcctgccatc tgtgctcttc gtcatctgac
     2161 cagccggcat caggaagccg agatggccca gaatgccgtt cgccttcatt atggactgcc
     2221 tgttgtggtt aaactcctgc acccaccatc ccactggcct ctgataaagg caactgttgg
     2281 attgattcga aaccttgccc tttgcccagc aaatcatgcg cctttgcggg aacagggtgc
     2341 tattccacga ctagttcagc tgcttgtacg agcacatcag gacacccaac ggcgcacctc
     2401 catgggtgga acgcagcagc agtttgtgga gggcgtgcgc atggaggaga tagtagaagg
     2461 gtgtactgga gctctccaca tccttgctcg ggacgttcac aaccggattg taatccgagg
     2521 actcaatacc attccattgt ttgtgcagtt gctttattct cccattgaaa atatccaaag
     2581 agtagctgca ggggtcctct gtgaacttgc tcaggacaag gaggctgcag aggccattga
     2641 agctgaggga gccacagctc ccctgacaga gttactccac tccaggaatg aaggcgtggc
     2701 aacatacgca gctgctgtcc tattccgaat gtctgaggac aagccacagg attacaagaa
     2761 gcggctttca gtcgagctga ccagttccct cttcaggaca gagccaatgg cttggaatga
     2821 gactgcagat cttggactgg acattggtgc ccagggagaa gcccttggat atcgccagga
     2881 tgatcccagc taccgttctt ttcactctgg tggatacggc caggatgcct tggggatgga
     2941 ccctatgatg gagcatgaga tgggtggcca ccaccctggt gctgactatc cagttgatgg
     3001 gctgcctgat ctgggacacg cccaggacct catggatggg ctgcccccag gtgatagcaa
     3061 tcagctggcc tggtttgata ctgacctgta aaagcttgga tccaccggat ctagataact
     3121 gatc
//
