LOCUS       sequence-89012-e        2201 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane, Excitation: 551, Emission: 565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2201)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKO2-CD36-C-10
FEATURES             Location/Qualifiers
     source          1..2201
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             35..688
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtcgc caccatggtg agtgtgatta aaccagagat
       61 gaagatgagg tactacatgg acggctccgt caatgggcat gagttcacaa ttgaaggtga
      121 aggcacaggc agaccttacg agggacatca agagatgaca ctacgcgtca caatggccga
      181 gggcgggcca atgcctttcg cgtttgactt agtgtcacac gtgttctgtt acggccacag
      241 agtatttact aaatatccag aagagatacc agactatttc aaacaagcat ttcctgaagg
      301 cctgtcatgg gaaaggtcgt tggagttcga agatggtggg tccgcttcag tcagtgcgca
      361 tataagcctt agaggaaaca ccttctacca caaatccaaa tttactgggg ttaactttcc
      421 tgccgatggt cctatcatgc aaaaccaaag tgttgattgg gagccatcaa ccgagaaaat
      481 tactgccagc gacggagttc tgaagggtga tgttacgatg tacctaaaac ttgaaggagg
      541 cggcaatcac aaatgccaaa tgaagactac ttacaaggcg gcaaaagaga ttcttgaaat
      601 gccaggagac cattacatcg gccatcgcct cgtcaggaaa accgaaggca acattactga
      661 gcaggtagaa gatgcagtag ctcatagctc cggactcaga tctggcagca gtggaggtgg
      721 ctgtgaccgg aactgtgggc tcatcgctgg ggctgtcatt ggtgctgtcc tggctgtgtt
      781 tggaggtatt ctaatgccag ttggagacct gcttatccag aagacaatta aaaagcaagt
      841 tgtcctcgaa gaaggtacaa ttgcttttaa aaattgggtt aaaacaggca cagaagttta
      901 cagacagttt tggatctttg atgtgcaaaa tccacaggaa gtgatgatga acagcagcaa
      961 cattcaagtt aagcaaagag gtccttatac gtacagagtt cgttttctag ccaaggaaaa
     1021 tgtaacccag gacgctgagg acaacacagt ctctttcctg cagcccaatg gtgccatctt
     1081 cgaaccttca ctatcagttg gaacagaggc tgacaacttc acagttctca atctggctgt
     1141 ggcagctgca tcccatatct atcaaaatca atttgttcaa atgatcctca attcacttat
     1201 taacaagtca aaatcttcta tgttccaagt cagaactttg agagaactgt tatggggcta
     1261 tagggatcca tttttgagtt tggttccgta ccctgttact accacagttg gtctgtttta
     1321 tccttacaac aatactgcag atggagttta taaagttttc aatggaaaag ataacataag
     1381 taaagttgcc ataatcgaca catataaagg taaaaggaat ctgtcctatt gggaaagtca
     1441 ctgcgacatg attaatggta cagatgcagc ctcatttcca ccttttgttg agaaaagcca
     1501 ggtattgcag ttcttttctt ctgatatttg caggtcaatc tatgctgtat ttgaatccga
     1561 cgttaatctg aaaggaatcc ctgtgtatag atttgttctt ccatccaagg cctttgcctc
     1621 tccagttgaa aacccagaca actattgttt ctgcacagaa aaaattatct caaaaaattg
     1681 tacatcatat ggtgtgctag acatcagcaa atgcaaagaa gggagacctg tgtacatttc
     1741 acttcctcat tttctgtatg caagtcctga tgtttcagaa cctattgatg gattaaaccc
     1801 aaatgaagaa gaacatagga catacttgga tattgaacct ataactggat tcactttaca
     1861 atttgcaaaa cggctgcagg tcaacctatt ggtcaagcca tcagaaaaaa ttcaagtatt
     1921 aaagaatctg aagaggaact atattgtgcc tattctttgg cttaatgaga ctgggaccat
     1981 tggtgatgag aaggcaaaca tgttcagaag tcaagtaact ggaaaaataa acctccttgg
     2041 cctgatagaa atgatcttac tcagtgttgg tgtggtgatg tttgttgctt ttatgatttc
     2101 atattgtgca tgcagatcga aaacaataaa ataagaattc tgcagtcgac ggtaccgcgg
     2161 gcccgggatc caccggatct agataactga tcataatcag c
//
