LOCUS       sequence-89014-e        1290 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Centromeres, Excitation: 551, Emission: 565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1290)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 1290)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKO2-CENPB-N-22
FEATURES             Location/Qualifiers
     source          1..1290
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      16..25
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    529..581
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      589..598
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             595..1251
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
ORIGIN
        1 acgtcagatc cgctagccac catgggcccc aagcggcggc agctgacgtt ccgggagaag
       61 tcgcggatca tccaggaggt ggaggagaac ccggacctac gcaagggcga gatcgcgcgg
      121 cgcttcaaca tcccgccgtc cacgctgagc acgatcctga agaacaagcg cgccatcctg
      181 gcgtcggagc gcaagtacgg ggtggcctcc acctgccgca agaccaacaa gctgtctccc
      241 tacgacaagc tcgagggctt gctcatcgcc tggttccagc agatccgcgc cgccggcctg
      301 ccggtcaagg gcatcatcct caaggagaag gcgctgcgca tagccgagga gctgggcatg
      361 gacgacttca ccgcctccaa cggctggctg gaccgcttcc gccggcgcca cggcgtggtg
      421 tcctgcagcg gcgtggcccg cgcccgcgcg cgaaacgctg ccccccgcac cccggcggcg
      481 cctgccagtc cggccgcggt gccctcggag ggcagtggcg ggagcactct cgagctcaag
      541 cttcgaattc tgcagtcgac ggtaccgcgg gcccgggatc caccggtcgc caccatggtg
      601 agtgtgatta aaccagagat gaagatgagg tactacatgg acggctccgt caatgggcat
      661 gagttcacaa ttgaaggtga aggcacaggc agaccttacg agggacatca agagatgaca
      721 ctacgcgtca caatggccga gggcgggcca atgcctttcg cgtttgactt agtgtcacac
      781 gtgttctgtt acggccacag agtatttact aaatatccag aagagatacc agactatttc
      841 aaacaagcat ttcctgaagg cctgtcatgg gaaaggtcgt tggagttcga agatggtggg
      901 tccgcttcag tcagtgcgca tataagcctt agaggaaaca ccttctacca caaatccaaa
      961 tttactgggg ttaactttcc tgccgatggt cctatcatgc aaaaccaaag tgttgattgg
     1021 gagccatcaa ccgagaaaat tactgccagc gacggagttc tgaagggtga tgttacgatg
     1081 tacctaaaac ttgaaggagg cggcaatcac aaatgccaaa tgaagactac ttacaaggcg
     1141 gcaaaagaga ttcttgaaat gccaggagac cattacatcg gccatcgcct cgtcaggaaa
     1201 accgaaggca acattactga gcaggtagaa gatgcagtag ctcatagcta agcggccgcg
     1261 actctagatc ataatcagcc ataccacata
//
