LOCUS       sequence-89015-e        1415 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Clathrin Vesicles, Excitation: 551, Emission: 565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1415)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 1415)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKO2-Clathrin-15
FEATURES             Location/Qualifiers
     source          1..1415
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             34..687
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
ORIGIN
        1 acgtcagatc cgctagcgct accggtcgcc accatggtga gtgtgattaa accagagatg
       61 aagatgaggt actacatgga cggctccgtc aatgggcatg agttcacaat tgaaggtgaa
      121 ggcacaggca gaccttacga gggacatcaa gagatgacac tacgcgtcac aatggccgag
      181 ggcgggccaa tgcctttcgc gtttgactta gtgtcacacg tgttctgtta cggccacaga
      241 gtatttacta aatatccaga agagatacca gactatttca aacaagcatt tcctgaaggc
      301 ctgtcatggg aaaggtcgtt ggagttcgaa gatggtgggt ccgcttcagt cagtgcgcat
      361 ataagcctta gaggaaacac cttctaccac aaatccaaat ttactggggt taactttcct
      421 gccgatggtc ctatcatgca aaaccaaagt gttgattggg agccatcaac cgagaaaatt
      481 actgccagcg acggagttct gaagggtgat gttacgatgt acctaaaact tgaaggaggc
      541 ggcaatcaca aatgccaaat gaagactact tacaaggcgg caaaagagat tcttgaaatg
      601 ccaggagacc attacatcgg ccatcgcctc gtcaggaaaa ccgaaggcaa cattactgag
      661 caggtagaag atgcagtagc tcatagctcc ggactcagat ctcgagctca agcttcgaat
      721 tctgcagtcg acatggctga tgactttggc ttcttctcgt cgtcggagag tggtgccccg
      781 gaggcggcgg aggaggaccc ggcggccgcc ttcctggccc agcaggagag cgagattgca
      841 ggcatagaga acgacgaggg cttcggggca cctgccggca gccatgcggc ccccgcacag
      901 ccgggcccca cgagtggggc tggttctgag gacatgggga ccacagtcaa tggagatgtg
      961 tttcaggagg ccaacggtcc tgctgatggc tacgcagcca ttgcccaggc tgacaggctg
     1021 acccaggagc ctgagagcat ccgcaagtgg cgagaggagc agaggaaacg gctgcaagag
     1081 ctggatgctg catctaaggt cacggaacag gaatggcggg agaaggccaa gaaggacctg
     1141 gaggagtgga accagcgcca gagtgaacaa gtagagaaga acaagatcaa caaccgggca
     1201 tccgaggagg ctttcgtgaa ggaatccaag gaggagaccc caggcacaga gtgggagaag
     1261 gtggcccagc tatgtgactt caaccccaag agcagcaagc agtgcaaaga tgtgtcccgc
     1321 ctgcgctcgg tgctcatgtc cctgaagcag acgccactgt cccgctaggg atccaccgga
     1381 tctagataac tgatcataat cagccatacc acatt
//
