LOCUS       sequence-89023-e        1611 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: MT End Binding Protein, Excitation: 551, Emission: 
            565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1611)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKO2-EB3-7
FEATURES             Location/Qualifiers
     source          1..1611
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      43..52
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      907..916
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             913..1569
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
ORIGIN
        1 gactcagatc tcgagctcaa gcttcgaatt ctgcagtcga ctgccaccat ggccgtcaat
       61 gtgtactcca catctgtgac cagtgaaaat ctgagtcgcc atgatatgct tgcatgggtc
      121 aacgactccc tgcacctcaa ctataccaag atagaacagc tttgttcagg ggcagcctac
      181 tgccagttca tggacatgct cttccccggc tgtgtgcact tgaggaaagt gaagttccag
      241 gccaaactag agcatgaata catccacaac ttcaaggtgc tgcaagcagc tttcaagaag
      301 atgggtgttg acaaaatcat tcctgtagag aaattagtga aaggaaaatt ccaagataat
      361 tttgagttta ttcagtggtt taagaaattc tttgacgcaa actatgatgg aaaggattac
      421 aaccctctgc tggcgcggca gggccaggac gtagcgccac ctcctaaccc aggtgatcag
      481 atcttcaaca aatccaagaa actcattggc acagcagttc cacagaggac gtcccccaca
      541 ggcccaaaaa acatgcagac ctctggccgg ctgagcaatg tggccccccc ctgcattctc
      601 cggaagaatc ctccatcagc ccgaaatggc ggccatgaga ctgatgccca aattcttgaa
      661 ctcaaccaac agctggtgga cttgaagctg acagtggatg ggctggagaa ggaacgtgac
      721 ttctacttca gcaaacttcg tgacatcgag ctcatctgcc aggagcatga aagtgaaaac
      781 agccctgtta tctcaggcat cattggcatc ctctatgcca cagaggaagg attcgcaccc
      841 cctgaggacg atgagattga agagcatcaa caagaagacc aggacgagta cctggatcca
      901 ccggtcgcca ccatggtgag tgtgattaaa ccagagatga agatgaggta ctacatggac
      961 ggctccgtca atgggcatga gttcacaatt gaaggtgaag gcacaggcag accttacgag
     1021 ggacatcaag agatgacact acgcgtcaca atggccgagg gcgggccaat gcctttcgcg
     1081 tttgacttag tgtcacacgt gttctgttac ggccacagag tatttactaa atatccagaa
     1141 gagataccag actatttcaa acaagcattt cctgaaggcc tgtcatggga aaggtcgttg
     1201 gagttcgaag atggtgggtc cgcttcagtc agtgcgcata taagccttag aggaaacacc
     1261 ttctaccaca aatccaaatt tactggggtt aactttcctg ccgatggtcc tatcatgcaa
     1321 aaccaaagtg ttgattggga gccatcaacc gagaaaatta ctgccagcga cggagttctg
     1381 aagggtgatg ttacgatgta cctaaaactt gaaggaggcg gcaatcacaa atgccaaatg
     1441 aagactactt acaaggcggc aaaagagatt cttgaaatgc caggagacca ttacatcggc
     1501 catcgcctcg tcaggaaaac cgaaggcaac attactgagc aggtagaaga tgcagtagct
     1561 catagctaag cggccgcgac tctagatcat aatcagccat accacattga g
//
