LOCUS       sequence-89030-e        2523 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nuclear Envelope, Excitation: 551, Emission: 565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2523)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKO2-LaminB1-10
FEATURES             Location/Qualifiers
     source          1..2523
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             35..688
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtcgc caccatggtg agtgtgatta aaccagagat
       61 gaagatgagg tactacatgg acggctccgt caatgggcat gagttcacaa ttgaaggtga
      121 aggcacaggc agaccttacg agggacatca agagatgaca ctacgcgtca caatggccga
      181 gggcgggcca atgcctttcg cgtttgactt agtgtcacac gtgttctgtt acggccacag
      241 agtatttact aaatatccag aagagatacc agactatttc aaacaagcat ttcctgaagg
      301 cctgtcatgg gaaaggtcgt tggagttcga agatggtggg tccgcttcag tcagtgcgca
      361 tataagcctt agaggaaaca ccttctacca caaatccaaa tttactgggg ttaactttcc
      421 tgccgatggt cctatcatgc aaaaccaaag tgttgattgg gagccatcaa ccgagaaaat
      481 tactgccagc gacggagttc tgaagggtga tgttacgatg tacctaaaac ttgaaggagg
      541 cggcaatcac aaatgccaaa tgaagactac ttacaaggcg gcaaaagaga ttcttgaaat
      601 gccaggagac cattacatcg gccatcgcct cgtcaggaaa accgaaggca acattactga
      661 gcaggtagaa gatgcagtag ctcatagctc cggactcaga tctcgagctc aagcttcgat
      721 ggcgactgcg acccccgtgc cgccgcggat gggcagccgc gctggcggcc ccaccacgcc
      781 gctgagcccc acgcgcctgt cgcggctcca ggagaaggag gagctgcgcg agctcaatga
      841 ccggctggcg gtgtacatcg acaaggtgcg cagcctggag acggagaaca gcgcgctgca
      901 gctgcaggtg acggagcgcg aggaggtgcg cggccgtgag ctcaccggcc tcaaggcgct
      961 ctacgagacc gagctggccg acgcgcgacg cgcgctcgac gacacggccc gcgagcgcgc
     1021 caagctgcag atcgagctgg gcaagtgcaa ggcggaacac gaccagctgc tcctcaacta
     1081 tgctaagaag gaatctgatc ttaatggcgc ccagatcaag cttcgagaat atgaagcagc
     1141 actcaattcg aaagatgcag ctcttgctac tgcacttggt gacaaaaaaa gtttagaggg
     1201 agatttggag gatctgaagg atcagattgc ccagttggaa gcctccttag ctgcagccaa
     1261 aaaacagtta gcagatgaaa ctttacttaa agtagatttg gagaatcgtt gtcagagcct
     1321 tactgaggac ttggagtttc gcaaaagcat gtatgaagag gagattaacg agaccagaag
     1381 gaagcatgaa acgcgcttgg tagaggtgga ttctgggcgt caaattgagt atgagtacaa
     1441 gctggcgcaa gcccttcatg agatgagaga gcaacatgat gcccaagtga ggctgtataa
     1501 ggaggagctg gagcagactt accatgccaa acttgagaat gccagactgt catcagagat
     1561 gaatacttct actgtcaaca gtgccaggga agaactgatg gaaagccgca tgagaattga
     1621 gagcctttca tcccagcttt ctaatctaca gaaagagtct agagcatgtt tggaaaggat
     1681 tcaagaatta gaggacttgc ttgctaaaga aaaagacaac tctcgtcgca tgctgacaga
     1741 caaagagaga gagatggcgg aaataaggga tcaaatgcag caacagctga atgactatga
     1801 acagcttctt gatgtaaagt tagccctgga catggaaatc agtgcttaca ggaaactctt
     1861 agaaggcgaa gaagagaggt tgaagctgtc tccaagccct tcttcccgtg tgacagtatc
     1921 ccgagcatcc tcaagtcgta gtgtacgtac aactagagga aagcggaaga gggttgatgt
     1981 ggaagaatca gaggcgagta gtagtgttag catctctcat tccgcctcag ccactggaaa
     2041 tgtttgcatc gaagaaattg atgttgatgg gaaatttatc cgcttgaaga acacttctga
     2101 acaggatcaa ccaatgggag gctgggagat gatcagaaaa attggagaca catcagtcag
     2161 ttataaatat acctcaagat atgtgctgaa ggcaggccag actgttacaa tttgggctgc
     2221 aaacgctggt gtcacagcca gccccccaac tgacctcatc tggaagaacc agaactcgtg
     2281 gggcactggc gaagatgtga aggttatatt gaaaaattct cagggagagg aggttgctca
     2341 aagaagtaca gtctttaaaa caaccatacc tgaagaagag gaggaggagg aagaagcagc
     2401 tggagtggtt gttgaggaag aacttttcca ccagcaggga accccaagag catccaatag
     2461 aagctgtgca attatgtagg gatccaccgg atctagataa ctgatcataa tcagccatac
     2521 cac
//
