LOCUS       sequence-89056-e        5985 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Tight Junctions, Excitation: 551, Emission: 565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5985)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 5985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKO2-ZO1-C-14
FEATURES             Location/Qualifiers
     source          1..5985
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             35..688
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
     RBS             2389..2397
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 accgtcagat ccgctagcgc taccggtcgc caccatggtg agtgtgatta aaccagagat
       61 gaagatgagg tactacatgg acggctccgt caatgggcat gagttcacaa ttgaaggtga
      121 aggcacaggc agaccttacg agggacatca agagatgaca ctacgcgtca caatggccga
      181 gggcgggcca atgcctttcg cgtttgactt agtgtcacac gtgttctgtt acggccacag
      241 agtatttact aaatatccag aagagatacc agactatttc aaacaagcat ttcctgaagg
      301 cctgtcatgg gaaaggtcgt tggagttcga agatggtggg tccgcttcag tcagtgcgca
      361 tataagcctt agaggaaaca ccttctacca caaatccaaa tttactgggg ttaactttcc
      421 tgccgatggt cctatcatgc aaaaccaaag tgttgattgg gagccatcaa ccgagaaaat
      481 tactgccagc gacggagttc tgaagggtga tgttacgatg tacctaaaac ttgaaggagg
      541 cggcaatcac aaatgccaaa tgaagactac ttacaaggcg gcaaaagaga ttcttgaaat
      601 gccaggagac cattacatcg gccatcgcct cgtcaggaaa accgaaggca acattactga
      661 gcaggtagaa gatgcagtag ctcatagctc cggaagtgct gggagcgcaa gcggaagcgg
      721 aggctccggt gaggaaacag ctatatggga acaacataca gtgacgcttc acagggctcc
      781 tggatttgga tttggaattg caatatctgg tggacgagat aatcctcatt ttcagagtgg
      841 ggaaacgtca atagtgattt cagatgtgct gaaaggagga ccagctgaag gacagctaca
      901 ggaaaatgac cgagttgcaa tggttaacgg agtttcaatg gataatgttg aacatgcttt
      961 tgctgttcag caactaagga aaagtgggaa aaatgcaaaa attacaatta gaaggaagaa
     1021 gaaagttcaa ataccagtaa gtcgtcctga tcctgaacca gtatctgata atgaagaaga
     1081 tagttatgat gaggaaatac atgatccaag aagtggccgg agtggtgtgg ttaacagaag
     1141 gagtgagaag atttggccga gggatagaag tgcaagtaga gagaggagct tgtccccgcg
     1201 gtcagacagg cggtcagtgg cttccagcca gcctgctaaa cctactaaag tcacactggt
     1261 gaaatcccgg aaaaatgaag aatatggtct tcgattggca agccatatat ttgttaagga
     1321 aatttcacaa gatagtttgg cagcaagaga tggcaatatt caagaaggtg atgttgtatt
     1381 gaagataaat ggtactgtga cagaaaatat gtcattgaca gatgcaaaga cattgataga
     1441 aaggtctaaa ggcaaattaa aaatggtagt tcaaagagat gaacgggcta cgctattgaa
     1501 tgtccctgat ctttctgaca gcatccactc tgctaatgcc tctgagagag acgacatttc
     1561 agaaattcag tcactggcat cagatcattc tggtcgatca cacgataggc ctccccgccg
     1621 cagccggtca cgatctcctg accagcggtc agagccttct gatcattcca ggcactcgcc
     1681 gcagcagcca agcaatggca gtctgcggag tagagatgaa gagagaattt ctaaacctgg
     1741 ggctgtctca actcctgtaa agcatgctga tgatcacaca cctaaaacag tggaagaagt
     1801 tacagttgaa agaaatgaga aacaaacacc ttctcttcca gaaccaaagc ctgtgtatgc
     1861 ccaagttggc aaccagatgt ggatttacct gtcagtccat ctgatggtgt cctacctaat
     1921 tcaactcatg aagatgggat ttcttcggcc cagcatgaaa ttggtaaaat tcagaaaagg
     1981 agatagtgtg ggtttgcggc tggctggtgg aaatgatgtt ggaatatttg tagctggcgt
     2041 tctagaagat agccctgcag ccaaggaagg cttagaggaa ggtgatcaaa ttctcagggt
     2101 aaacaacgta gattttacaa atatcataag agaagaagcc gtccttttcc tgcttgacct
     2161 ccctaaagga gaagaagtga ccatattggc tcagaagaag aaggatgttt atcgtcgcat
     2221 tgtagaatca gatgtaggag attctttcta tattagaacc cattttgaat atgaaaagga
     2281 atctccctat ggacttagtt ttaacaaagg agaggtgttc cgtgctgtgg ataccttgta
     2341 caatggaaaa ctgggctctt ggcttgctat tcgaattggt aaaaatcata aggaggtaga
     2401 acgaggcatc atccctaata agaacagagc tgagcagctc gccagtgtac agtatacact
     2461 tccaaaaaca gcaggcggag accgtgctga cttctggaga ttcagaggtc ttcgcagctc
     2521 caagagaaat cttcgaaaaa gcagagagga tttgtccgct cagcctgttc aaacaaagtt
     2581 tccagcttat gaaagagtgg ttcttcgaga agctggattt ctgaggcctg taaccatttt
     2641 tggaccaata gctgatgttg ccagagaaaa gctggcaaga gaagaaccag atatttatca
     2701 aattgcaaag agtgaaccac gagacgctgg aactgaccaa cgtagctctg gctatattcg
     2761 cctgcataca ataaagcaaa tcatagatca agacaaacat gctttattag atgtaacacc
     2821 aaatgcagtt gatcgtctta actatgccca gtggtatcca attgttgtat ttcttaaccc
     2881 tgattctaag caaggagtaa aaacaatgag aatgaggtta tgtccagaat ctcggaaaag
     2941 tgccaggaag ttatacgagc gatctcataa acttgctaaa aataatcacc atctttttac
     3001 aactacaatt aacttaaatt caatgaatga tggttggtat ggtgcgctga aagaagcagt
     3061 tcaacaacag caaaaccagc tggtatgggt ttccgaggga aaggcggatg gtgctacaag
     3121 tgatgacctt gatttgcatg atgatcgtct gtcctacctg tcagctccag gtagtgaata
     3181 ctcaatgtat agcacggaca gtagacacac ttctgactat gaagacacag acacagaagg
     3241 cggggcctac actgatcaag aactagatga aactcttaat gatgaggttg ggactccacc
     3301 ggagtctgcc attacacggt cctctgagcc tgtaagagag gactcctctg gaatgcatca
     3361 tgaaaaccaa acatatcctc cttactcacc acaagcgcag ccacaaccaa ttcatagaat
     3421 agactcccct ggatttaagc cagcctctca acagaaagca gaagcttcat ctccagtccc
     3481 ttacctttcg cctgaaacaa acccagcatc atcaacctct gctgttaatc ataatgtaaa
     3541 tttaactaat gtcagactgg aggagcccac cccagctcct tccacctctt actcaccaca
     3601 agctgattct ttaagaacac caagtactga ggcagctcac ataatgctaa gagatcaaga
     3661 accatcattg tcgtcgcatg tagatccaac aaaggtgtat agaaaggacc catatcccga
     3721 ggaaatgatg aggcagaacc atgttttgaa acagccagcc gttagtcacc cagggcacag
     3781 gccagacaaa gagcctaatc tgacctatga accccaactc ccatacgtag agaaacaagc
     3841 cagcagagac ctggagcagc ccacatacag atacgagtcc tcaagctata cggaccagtt
     3901 ttctcgaaac tatgaacatc gtctgcgata cgaagatcgc gtccccatgt atgaagaaca
     3961 gtggtcatat tatgatgaca aacagcccta cccatctcgg ccaccttttg ataatcagca
     4021 ctctcaagac cttgactcca gacagcatcc cgaagagtcc tcagaacgag ggtactttcc
     4081 acgttttgaa gagccagccc ctctgtctta cgacagcaga ccacgttacg aacaggcacc
     4141 tagagcatcc gccctgcggc acgaagagca gccagctcct gggtatgaca cacatggtag
     4201 actcagaccg gaagcccagc cccacccttc agcagggccc aagcctgcag agtccaagca
     4261 gtattttgag caatattcac gcagttacga gcaagtacca ccccaaggat ttacctctag
     4321 agcaggtcat tttgagcctc tccatggtgc tgcagctgtc cctccgctga taccttcatc
     4381 tcagcataag ccagaagctc tgccttcaaa caccaaacca ctgcctccac ccccaactca
     4441 aaccgaagaa gaggaagatc cagcaatgaa gccacagtct gtactcacca gagttaagat
     4501 gtttgaaaac aaaaggtctg catccttaga gaccaagaag gatgtaaatg acactggcag
     4561 ttttaagcct ccagaagtag catctaaacc ttcaggtgct cccatcattg gtcccaaacc
     4621 cacttctcag aatcaattca gtgaacatga caaaactctg tacaggattc cagaacctca
     4681 aaaacctcaa ctgaagccac ctgaagatat tgttcggtcc aatcattatg accctgaaga
     4741 agatgaagaa tattatcgaa aacagctgtc atactttgac cgaagaagtt ttgagaataa
     4801 gcctcctgca cacattgctg ccagccatct ctccgagcct gcaaagccag ctcattctca
     4861 gaatcaatca aatttttcta gttattcttc aaagggaaag cctcctgaag ctgatggtgt
     4921 ggatagatca tttggcgaga aacgctatga acccatccag gccactcccc ctcctcctcc
     4981 attgccctcg cagtatgccc agccatctca gcctgtcacc agcgcgtctc tccacataca
     5041 ttctaaggga gcacatggtg aaggtaattc agtgtcattg gattttcaga attccttagt
     5101 gtccaaacca gacccacctc catctcagaa taagccagca actttcagac caccaaaccg
     5161 agaagatact gctcaggcag ctttctatcc ccagaaaagt tttccagata aagccccagt
     5221 taatggaact gaacagactc agaaaacagt cactccagca tacaatcgat tcacaccaaa
     5281 accatataca agttctgccc gaccatttga acgcaagttt gaaagtccta aattcaatca
     5341 caatcttctg ccaagtgaaa ctgcacataa acctgacttg tcttcaaaaa ctcccacttc
     5401 tccaaaaact cttgtgaaat cgcacagttt ggcacagcct cctgagtttg acagtggagt
     5461 tgaaactttc tctatccatg cagagaagcc taaatatcaa ataaataata tcagcacagt
     5521 gcctaaagct attcctgtga gtccttcagc tgtggaagag gatgaagatg aagatggtca
     5581 tactgtggtg gccacagccc gaggcatatt taacagcaat gggggcgtgc tgagttccat
     5641 agaaactggt gttagtataa ttatccctca aggagccatt cccgaaggag ttgagcagga
     5701 aatctatttc aaggtctgcc gggacaacag catccttcca cctttagata aagagaaagg
     5761 tgaaacactg ctgagtcctt tggtgatgtg tggtccccat ggcctcaagt tcctgaagcc
     5821 tgtggagctg cgcttaccac actgtgatcc taaaacctgg caaaacaagt gtcttcccgg
     5881 agatccaaat tatctcgttg gagcaaactg tgtttctgtc cttattgacc acttttaagg
     5941 atccaccgga tctagataac tgatcataat cagccatacc acatt
//
