LOCUS       sequence-89082-e        1685 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus, Excitation: 548, Emission: 561.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1685)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 1685)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKOet-PCNA-19-SV40NLS-4
FEATURES             Location/Qualifiers
     source          1..1685
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      12..21
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             24..44
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             48..68
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             72..92
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             120..770
                     /codon_start=1
                     /product="monomeric Kusabira-Orange fluorescent protein 
                     (Karasawa et al., 2004)"
                     /label=mKO
                     /translation="MAVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRVT
                     MAKGGPMPFAFDLVSHVFCYGHRPFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSAS
                     VSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMYL
                     KLEGGGNHKCQFKTTYKAAKKILKMPGSHYISHRLVRKTEGNITELVEDAVAHS"
ORIGIN
        1 cagatccgct agccaccatg gatccaaaaa agaagagaaa ggtagatcca aaaaagaaga
       61 gaaaggtaga tccaaaaaag aagagaaagg taatggtgag caagggcgag gagaataaca
      121 tggccgtgat taaaccagag atgaagatga ggtactacat ggacggctcc gtcaatgggc
      181 atgagttcac aattgaaggt gaaggcacag gcagacctta cgagggacat caagagatga
      241 cactacgcgt cacaatggcc aagggcgggc caatgccttt cgcgtttgac ttagtgtcac
      301 acgtgttctg ttacggccac agacctttta ctaaatatcc agaagagata ccagactatt
      361 tcaaacaagc atttcctgaa ggcctgtcat gggaaaggtc gttggagttc gaagatggtg
      421 ggtccgcttc agtcagtgcg catataagcc ttagaggaaa caccttctac cacaaatcca
      481 aatttactgg ggttaacttt cctgccgatg gtcctatcat gcaaaaccaa agtgttgatt
      541 gggagccatc aaccgagaaa attactgcca gcgacggagt tctgaagggt gatgttacga
      601 tgtacctaaa acttgaagga ggcggcaatc acaaatgcca attcaagact acttacaagg
      661 cggcaaaaaa gattcttaaa atgccaggaa gccattacat cagccatcgc ctcgtcagga
      721 aaaccgaagg caacattact gagctggtag aagatgcagt agctcattcc ggcggcatgg
      781 acgagctgta caagtccgga gaagggcaag ggcaagggca agggccgggc cgcggctacg
      841 cgtatcgatc catgttcgag gcgcgcctgg tccagggctc catcctcaag aaggtgttgg
      901 aggcactcaa ggacctcatc aacgaggcct gctgggatat tagctccagc ggtgtaaacc
      961 tgcagagcat ggactcgtcc cacgtctctt tggtgcagct caccctgcgg tctgagggct
     1021 tcgacaccta ccgctgcgac cgcaacctgg ccatgggcgt gaacctcacc agtatgtcca
     1081 aaatactaaa atgcgccggc aatgaagata tcattacact aagggccgaa gataacgcgg
     1141 ataccttggc gctagtattt gaagcaccaa accaggagaa agtttcagac tatgaaatga
     1201 agttgatgga tttagatgtt gaacaacttg gaattccaga acaggagtac agctgtgtag
     1261 taaagatgcc ttctggtgaa tttgcacgta tatgccgaga tctcagccat attggagatg
     1321 ctgttgtaat ttcctgtgca aaagacggag tgaaattttc tgcaagtgga gaacttggaa
     1381 atggaaacat taaattgtca cagacaagta atgtcgataa agaggaggaa gctgttacca
     1441 tagagatgaa tgaaccagtt caactaactt ttgcactgag gtacctgaac ttctttacaa
     1501 aagccactcc actctcttca acggtgacac tcagtatgtc tgcagatgta ccccttgttg
     1561 tagagtataa aattgcggat atgggacact taaaatacta cttggctccc aagatcgagg
     1621 atgaagaagg atcttaggga tccaccggat ctagataact gatcataatc agccatacca
     1681 cattt
//
