LOCUS       sequence-89083-e        1236 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytoskeleton, Excitation: 548, Emission: 561.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1236)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 1236)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKOet-Profilin-10
FEATURES             Location/Qualifiers
     source          1..1236
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             62..712
                     /codon_start=1
                     /product="monomeric Kusabira-Orange fluorescent protein 
                     (Karasawa et al., 2004)"
                     /label=mKO
                     /translation="MAVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRVT
                     MAKGGPMPFAFDLVSHVFCYGHRPFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSAS
                     VSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMYL
                     KLEGGGNHKCQFKTTYKAAKKILKMPGSHYISHRLVRKTEGNITELVEDAVAHS"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg aggagaataa
       61 catggccgtg attaaaccag agatgaagat gaggtactac atggacggct ccgtcaatgg
      121 gcatgagttc acaattgaag gtgaaggcac aggcagacct tacgagggac atcaagagat
      181 gacactacgc gtcacaatgg ccaagggcgg gccaatgcct ttcgcgtttg acttagtgtc
      241 acacgtgttc tgttacggcc acagaccttt tactaaatat ccagaagaga taccagacta
      301 tttcaaacaa gcatttcctg aaggcctgtc atgggaaagg tcgttggagt tcgaagatgg
      361 tgggtccgct tcagtcagtg cgcatataag ccttagagga aacaccttct accacaaatc
      421 caaatttact ggggttaact ttcctgccga tggtcctatc atgcaaaacc aaagtgttga
      481 ttgggagcca tcaaccgaga aaattactgc cagcgacgga gttctgaagg gtgatgttac
      541 gatgtaccta aaacttgaag gaggcggcaa tcacaaatgc caattcaaga ctacttacaa
      601 ggcggcaaaa aagattctta aaatgccagg aagccattac atcagccatc gcctcgtcag
      661 gaaaaccgaa ggcaacatta ctgagctggt agaagatgca gtagctcatt ccggcggcat
      721 ggacgagctg tacaagtccg gactcagatc tcgagctcaa gcttccatgg ccgggtggaa
      781 cgcctacatc gacagcctta tggcggacgg gacctgtcag gacgcggcca tcgtaggcta
      841 caaggactcg ccctccgtct gggccgccgt ccccgggaag accttcgtta gcattacgcc
      901 agctgaggtt ggtgtcctgg taggcaaaga ccggtcaagt tttttcgtca atgggctgac
      961 acttgggggc cagaaatgtt ctgtgatccg ggactcactg ctgcaagacg gggaatttac
     1021 aatggatctt cgtaccaaga gcaccggagg agcccccacc ttcaatgtca ctgtcaccat
     1081 gactgccaag acgctagtcc tgctgatggg caaagaaggt gtccacggtg gtttgatcaa
     1141 caagaaatgt tatgaaatgg cctctcacct gcggcgttcc cagtactaag gatccaccgg
     1201 atctagataa ctgatcataa tcagccatac cacatt
//
