LOCUS       sequence-89085-e        1915 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin/Focal Adhesions, Excitation: 548, Emission: 561.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1915)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 1915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKOet-VASP-5
FEATURES             Location/Qualifiers
     source          1..1915
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      27..36
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             60..710
                     /codon_start=1
                     /product="monomeric Kusabira-Orange fluorescent protein 
                     (Karasawa et al., 2004)"
                     /label=mKO
                     /translation="MAVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRVT
                     MAKGGPMPFAFDLVSHVFCYGHRPFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSAS
                     VSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMYL
                     KLEGGGNHKCQFKTTYKAAKKILKMPGSHYISHRLVRKTEGNITELVEDAVAHS"
ORIGIN
        1 cgtcagatcc gctagcgcta ccggtcgcca ccatggtgag caagggcgag gagaataaca
       61 tggccgtgat taaaccagag atgaagatga ggtactacat ggacggctcc gtcaatgggc
      121 atgagttcac aattgaaggt gaaggcacag gcagacctta cgagggacat caagagatga
      181 cactacgcgt cacaatggcc aagggcgggc caatgccttt cgcgtttgac ttagtgtcac
      241 acgtgttctg ttacggccac agacctttta ctaaatatcc agaagagata ccagactatt
      301 tcaaacaagc atttcctgaa ggcctgtcat gggaaaggtc gttggagttc gaagatggtg
      361 ggtccgcttc agtcagtgcg catataagcc ttagaggaaa caccttctac cacaaatcca
      421 aatttactgg ggttaacttt cctgccgatg gtcctatcat gcaaaaccaa agtgttgatt
      481 gggagccatc aaccgagaaa attactgcca gcgacggagt tctgaagggt gatgttacga
      541 tgtacctaaa acttgaagga ggcggcaatc acaaatgcca attcaagact acttacaagg
      601 cggcaaaaaa gattcttaaa atgccaggaa gccattacat cagccatcgc ctcgtcagga
      661 aaaccgaagg caacattact gagctggtag aagatgcagt agctcattcc ggcggcatgg
      721 acgagctgta caagtccgga ctcagatcta tgagcgagac ggtcatctgt tccagccggg
      781 ctactgtgat gctttatgat gacagcaaca agcgatggct ccctgctggc actggtcccc
      841 aggccttcag ccgcgtccag atataccaca accccactgc taactccttc cgagttgttg
      901 gccgcaagat gcagccggat cagcaggtgg ttatcaactg tgccatcatt cggggtgtca
      961 agtacaatca ggccactccc atcttccatc agtggcgaga tgcccgccag gtctggggcc
     1021 tcaacttcgg cagcaaggag gacgccatac agtttgcaac aggcatggcc aacgccctag
     1081 aggccttgga aggaggtggg cctcccccag ccccagcacc ccctgcctgg tctgcccaga
     1141 atggtccctc cccagaggag ctggaacaac agaaaaggca gccggagcat atggagcgcc
     1201 gggtctccaa tgcaggaggc ccacctgctc ccccagctgg gggccctcct ccacctccag
     1261 gacctccccc tcctccaggt ccccccccac ccccaggtct gccctcctca ggggtatctg
     1321 gggcaggtca tggagcaggg gcagccccac cccctgcacc cccactccct acaacacagg
     1381 gccccaatag tgggggttcc ggggccccag gcctggctgc tgccattgct ggagccaaac
     1441 tcaggaaagt gagcaagcag gaggaggcct ctggggggcc cctggccccc aaagctgaga
     1501 acagtcgaag cactggtggg gggcttatgg aagagatgaa cgccatgctg gcccggagaa
     1561 gaaaagccac acaggttggg gagaagcccc ccaaagacga gtcagccagt gaggagtcag
     1621 aggcccgact ccctgcccag agtgaacctg tgagaagacc ctgggagaag aacagcacaa
     1681 ccttgccaag gatgaagtcg tcctcttctg tgactacctc cgaggcccac ccctcgacgc
     1741 cctgctccag tgatgactcc gacttggaga gggtgaagca ggagcttctg gaagaggtgc
     1801 ggaaggagct acagaaaatg aaagaggaaa tcatcgaagt ctttgtccag gagctgagga
     1861 agcggggttc tccttgagcg gccgcgactc tagatcataa tcagccatac cacat
//
