LOCUS       sequence-89094-e        1349 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Centromeres, Excitation: 549, Emission: 565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1349)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 1349)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mOrange2-CENPB-N-22
FEATURES             Location/Qualifiers
     source          1..1349
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    531..583
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      591..600
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             597..1307
                     /codon_start=1
                     /product="photostable monomeric orange derivative of DsRed 
                     fluorescent protein (Shaner et al., 2008)"
                     /label=mOrange2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPHFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GKIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
ORIGIN
        1 gtgacgtcag atccgctagc cccatgggcc ccaagcggcg gcagctgacg ttccgggaga
       61 agtcgcggat catccaggag gtggaggaga acccggacct acgcaagggc gagatcgcgc
      121 ggcgcttcaa catcccgccg tccacgctga gcacgatcct gaagaacaag cgcgccatcc
      181 tggcgtcgga gcgcaagtac ggggtggcct ccacctgccg caagaccaac aagctgtctc
      241 cctacgacaa gctcgagggc ttgctcatcg cctggttcca gcagatccgc gccgccggcc
      301 tgccggtcaa gggcatcatc ctcaaggaga aggcgctgcg catagccgag gagctgggca
      361 tggacgactt caccgcctcc aacggctggc tggaccgctt ccgccggcgc cacggcgtgg
      421 tgtcctgcag cggcgtggcc cgcgcccgcg cgcgaaacgc tgccccccgc accccggcgg
      481 cgcctgccag tccggccgcg gtgccctcgg agggcagtgg cgggagcact ctcgagctca
      541 agcttcgaat tctgcagtcg acggtaccgc gggcccggga tccaccggtc gccaccatgg
      601 tgagcaaggg cgaggagaat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc
      661 gcatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc
      721 cctacgaggg ctttcagacc gctaagctga aggtgaccaa gggtggcccc ctgcccttcg
      781 cctgggacat cctgtcccct catttcacct acggctccaa ggcctacgtg aagcaccccg
      841 ccgacatccc cgactacttc aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga
      901 tgaactacga ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg
      961 agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtgatgc
     1021 agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggtgccc
     1081 tgaagggcaa gatcaagatg aggctgaagc tgaaggacgg cggccactac acctccgagg
     1141 tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac atcgtcgaca
     1201 tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg
     1261 ccgagggccg ccactccacc ggcggcatgg acgagctgta caagtaagcg gccgcgactc
     1321 tagatcataa tcagccatac cacattgag
//
