LOCUS       sequence-89111-e        1252 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 549, Emission: 565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1252)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 1252)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mOrange2-H3-23
FEATURES             Location/Qualifiers
     source          1..1252
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      20..29
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    437..489
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      497..506
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             503..1213
                     /codon_start=1
                     /product="photostable monomeric orange derivative of DsRed 
                     fluorescent protein (Shaner et al., 2008)"
                     /label=mOrange2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPHFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GKIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
ORIGIN
        1 gacgtcagat ccgctagccg ccaccatggc tcgtactaaa cagacagctc ggaaatccac
       61 cggcggtaaa gcgccacgca agcagctggc taccaaggct gctcgcaaga gcgcgccggc
      121 taccggcggc gtgaaaaagc ctcaccgtta ccgcccgggc actgtggctc tgcgcgagat
      181 ccgccgctac caaaagtcga ccgagttgct gattcggaag ctgccgttcc agcgcctggt
      241 gcgagaaatc gcccaagact tcaagaccga tcttcgcttc cagagctctg cggtgatggc
      301 gctgcaggag gcttgtgagg cctacttggt agggctcttt gaggacacaa acctttgcgc
      361 catccatgct aagcgagtga ctattatgcc caaagacatc cagctcgctc gccgcattcg
      421 cggagaaaga gcggatctcg agctcaagct tcgaattctg cagtcgacgg taccgcgggc
      481 ccgggatcca ccggtcgcca ccatggtgag caagggcgag gagaataaca tggccatcat
      541 caaggagttc atgcgcttca aggtgcgcat ggagggctcc gtgaacggcc acgagttcga
      601 gatcgagggc gagggcgagg gccgccccta cgagggcttt cagaccgcta agctgaaggt
      661 gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcatt tcacctacgg
      721 ctccaaggcc tacgtgaagc accccgccga catccccgac tacttcaagc tgtccttccc
      781 cgagggcttc aagtgggagc gcgtgatgaa ctacgaggac ggcggcgtgg tgaccgtgac
      841 ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa
      901 cttcccctcc gacggccccg tgatgcagaa gaagaccatg ggctgggagg cctcctccga
      961 gcggatgtac cccgaggacg gtgccctgaa gggcaagatc aagatgaggc tgaagctgaa
     1021 ggacggcggc cactacacct ccgaggtcaa gaccacctac aaggccaaga agcccgtgca
     1081 gctgcccggc gcctacatcg tcgacatcaa gttggacatc acctcccaca acgaggacta
     1141 caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga
     1201 gctgtacaag taagcggccg cgactctaga tcataatcag ccataccaca tt
//
