LOCUS       sequence-89112-e        1151 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 549, Emission: 565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1151)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 1151)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mOrange2-H4-23
FEATURES             Location/Qualifiers
     source          1..1151
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    337..389
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      397..406
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             403..1113
                     /codon_start=1
                     /product="photostable monomeric orange derivative of DsRed 
                     fluorescent protein (Shaner et al., 2008)"
                     /label=mOrange2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPHFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GKIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
ORIGIN
        1 ccgtcagatc cgctagccgc caccatgtct ggccgcggca aaggcgggaa gggtcttggc
       61 aaaggcggcg ctaagcgcca ccgtaaagta ctgcgcgaca atatccaggg catcaccaag
      121 ccggccatcc ggcgccttgc tcgccgcggc ggcgtgaagc gcatctccgg cctcatctac
      181 gaggagactc gcggggtgct gaaggtgttc ctggagaacg tgatccggga cgccgtgacc
      241 tatacagagc acgccaagcg caagacggtc accgccatgg atgtggtcta cgcgctcaag
      301 cgccagggcc gcaccctcta cggtttcggt ggggatctcg agctcaagct tcgaattctg
      361 cagtcgacgg taccgcgggc ccgggatcca ccggtcgcca ccatggtgag caagggcgag
      421 gagaataaca tggccatcat caaggagttc atgcgcttca aggtgcgcat ggagggctcc
      481 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcttt
      541 cagaccgcta agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg
      601 tcccctcatt tcacctacgg ctccaaggcc tacgtgaagc accccgccga catccccgac
      661 tacttcaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa ctacgaggac
      721 ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag
      781 gtgaagctgc gcggcaccaa cttcccctcc gacggccccg tgatgcagaa gaagaccatg
      841 ggctgggagg cctcctccga gcggatgtac cccgaggacg gtgccctgaa gggcaagatc
      901 aagatgaggc tgaagctgaa ggacggcggc cactacacct ccgaggtcaa gaccacctac
      961 aaggccaaga agcccgtgca gctgcccggc gcctacatcg tcgacatcaa gttggacatc
     1021 acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac
     1081 tccaccggcg gcatggacga gctgtacaag taagcggccg cgactctaga tcataatcag
     1141 ccataccaca t
//
