LOCUS       sequence-89121-e        7164 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 549, Emission: 565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7164)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 7164)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mOrange2-Talin-R-15
FEATURES             Location/Qualifiers
     source          1..7164
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             34..741
                     /codon_start=1
                     /product="photostable monomeric orange derivative of DsRed 
                     fluorescent protein (Shaner et al., 2008)"
                     /label=mOrange2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPHFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GKIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
ORIGIN
        1 acgtcagatc cgctagcgct accggtcgcc accatggtga gcaagggcga ggagaataac
       61 atggccatca tcaaggagtt catgcgcttc aaggtgcgca tggagggctc cgtgaacggc
      121 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggctt tcagaccgct
      181 aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcat
      241 ttcacctacg gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttcaag
      301 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga actacgagga cggcggcgtg
      361 gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg
      421 cgcggcacca acttcccctc cgacggcccc gtgatgcaga agaagaccat gggctgggag
      481 gcctcctccg agcggatgta ccccgaggac ggtgccctga agggcaagat caagatgagg
      541 ctgaagctga aggacggcgg ccactacacc tccgaggtca agaccaccta caaggccaag
      601 aagcccgtgc agctgcccgg cgcctacatc gtcgacatca agttggacat cacctcccac
      661 aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc
      721 ggcatggacg agctgtacaa gtccggactc agatctcgag ctcaagctag cttcgaattc
      781 gccaccatgc agcagtacaa ccgagtgggg aaagtggagc acggctctgt ggctctgcca
      841 gccatcatgc gctctggagc ctctggtcct gagaatttcc aggtggggag catgccacct
      901 gcccagcagc agatcaccag tggccagatg caccgaggac acatgccacc tctgacttca
      961 gcccagcagg cgctcactgg aaccattaac tccagcatgc aggctgtgca ggctgcccag
     1021 gccactctgg atgactttga gactctaccc cctcttggcc aggatgctgc ctccaaagct
     1081 tggcgtaaga acaagatgga tgaatcaaag catgagatcc actcccaggt agatgccatc
     1141 acagctggca ctgcctctgt ggtaaacctg acagcaggag atcctgcaga gacagactat
     1201 acagcagtgg gctgtgcagt caccaccatc tcttccaacc tgacggagat gtcccgtggg
     1261 gtgaagctat tggctgccct gctggaggat gaaggcggca atggccggcc ccttctgcaa
     1321 gcagcaaagg gccttgcagg ggctgtgtcg gaactgcttc gcagtgctca gcccgccagc
     1381 gctgagcctc gtcagaacct gctgcaggca gccgggaacg tgggccaggc cagtggggag
     1441 ctgttgcagc aaattgggga aagtgacact gacccccact tccaggatgt tctaatgcag
     1501 ctagcaaacg cagtggcaag tgctgcagcc gccctggtcc tcaaggccaa gagtgtggcc
     1561 cagcgcacag aggattccgg gcttcagacc caagtgattg ctgcagctac gcaatgtgct
     1621 ctgtccactt cccagctggt ggcctgtacc aaggtggtag cacctacaat cagctcacct
     1681 gtgtgccaag aacagctagt cgaggctggg cgactggtgg ccaaagctgt ggagggctgt
     1741 gtgtctgcct cgcaagcagc tacagaagat ggacagctgc ttcgaggggt aggagcagca
     1801 gccacggctg tcacccaggc cctcaatgag ctgctgcagc acgtgaaggc ccacgccacc
     1861 ggagctgggc ctgccggtcg gtatgaccaa gccactgata ccatcctcac cgtcactgaa
     1921 aacatcttca gctccatggg tgatgctggg gagatggtgc gccaggcccg catcctggcc
     1981 caagccacct cagacctggt caatgctatc aaggccgatg ctgaggggga gagtgatctg
     2041 gagaactcta gaaagctcct gagtgctgcc aagatcctcg ctgatgccac cgccaagatg
     2101 gtggaggcgg ccaagggagc agccgcccac cctgacagtg aggaacagca gcagcgactg
     2161 cgtgaagcag ctgaggggct tcgcatggcc accaatgcag ctgcgcagaa cgccatcaag
     2221 aagaagttgg tgcagcgcct ggagcatgca gccaagcaag ctgcagcctc tgcaacacag
     2281 accattgctg cagcccaaca tgcagcctct gcccccaagg cctctgcggg cccccagccc
     2341 ctactggtgc agagctgtaa ggccgtggca gagcagattc cgttgctggt gcagggtgtc
     2401 cgaggaagtc aagctcagcc tgacagccct agtgctcagc tcgccctcat tgctgccagc
     2461 cagagcttcc tgcagccagg tgggaagatg gtggcagcgg caaaagcctc agtgccaaca
     2521 attcaggacc aggcttcagc gatgcagctg agtcagtgcg caaagaacct aggcactgcc
     2581 ctggccgagc tgcgcactgc tgctcagaag gctcaggagg catgtggacc tttggagatg
     2641 gattctgcac tgagtgttgt acaaaatcta gagaaagacc tacaggaaat aaaagcagca
     2701 gctcgagatg gcaagcttaa gcccttacct ggggaaacaa tggagaagtg tacccaagat
     2761 cttggcaaca gcaccaaagc ggtgagctct gccatcgcca agctgctggg ggagattgcc
     2821 cagggcaatg agaactatgc aggtattgca gctcgggatg tagctggtgg actaaggtca
     2881 ctagcccagg ctgcgcgggg tgtggctgcg ctgacatcag atcctgcagt gcaggccatt
     2941 gtgcttgaca cagccagcga cgttctggac aaggccagca gcctcattga agaggcaaaa
     3001 aaagcatctg gacacccagg ggacccagaa agccagcaaa ggcttgctca ggtagctaaa
     3061 gcagtgaccc aagccctgaa ccgctgtgtc agctgcctgc ctggccaaag agacgtggat
     3121 aatgccctac gagccgttgg agatgccagc aagcgactcc tgagtgactt gcttcctcca
     3181 agcacgggga catttcaaga agcacagagc cgattgaatg aagctgcagc tgggttaaat
     3241 caggccgcca cagaactggt gcaagcctcc cgaggaactc ctcaggacct ggctcgggcc
     3301 tccggccgat ttggacagga cttcagcacc ttcctggaag ctggtgtgga gatggccgga
     3361 caggccccga gccaggagga ccgagcccag gtggtgtcca acctgaaggg catatccatg
     3421 tcttcaagca aacttctcct tgccgctaag gccttgtcca cagaccctgc ttctcccaac
     3481 ctcaagagtc agctggctgc ggctgcccgg gcagtgacgg acagcatcaa ccagctcatc
     3541 accatgtgca cccagcaggc acctggccag aaggagtgtg acaatgcact tcggcagcta
     3601 gagacagtcc gagaactcct ggagaatcca gtccagccca tcaacgacat gtcctacttc
     3661 ggttgcttgg acagtgtcat ggagaactcc aaggtcctag gtgaggccat gactggcatc
     3721 tcccaaaatg ccaagaatgg aaatctgccg gagtttggag atgccattgc cacagcctcc
     3781 aaggctctct gtggcttcac ggaggcagcc gcacaggcag catatctagt tggtgtctct
     3841 gaccccaaca gccaagctgg acagcaagga ctggtggaac ccacacagtt tgcccgtgca
     3901 aaccaggcaa ttcagatggc ctgtcagagt ctgggggagc ctggctgtac ccaggcccag
     3961 gtgttatctg cagccactat tgtagccaaa cacacatctg cattgtgtaa cagctgtcgc
     4021 ctggcttccg ctaggactgc caatcccact gccaagcgcc agtttgtaca gtcagccaag
     4081 gaggtggcca acagtacagc caatcttgtc aagaccatca aggcactaga tggggacttc
     4141 acagaagaga accgtgccca gtgccgagca gccacagccc ctctgctgga agctgtggat
     4201 aacctgagtg cctttgcctc caaccctgag ttctccagcg tccctgccca gatcagccct
     4261 gagggcagag cagccatgga gcccattgta atctctgcta agacaatgtt ggagagtgct
     4321 ggagggctca tccagacagc tcgggcgtta gcagtcaatc cccgagaccc cccacgttgg
     4381 tctgtgctag ctggccactc tcggactgtc tcagattcca tcaagaaact tattacaagc
     4441 atgagggaca aagccccagg gcagctggag tgtgagacag ccattgcggc tctgaacagc
     4501 tgcttgcggg acctagatca ggcttcgctt gccgctgtca gccagcagct tgctccccgt
     4561 gaaggaatct ctcaagaggc tttgcacacc cagatgctca ctgcagtgca agaaatttct
     4621 catctcattg agccgctggc cagcgctgct cgggctgaag cctcccagct gggacacaag
     4681 gtgtcccaaa tggcccagta ctttgaacca ctcaccctgg ctgcagtggg tgctgcgtct
     4741 aagaccctga gccacccgca gcagatggca cttctggacc agactaaaac gttggcagag
     4801 tctgccttgc agttgctata tactgccaag gaggctggtg gtaaccccaa gcaagcagca
     4861 cacacccagg aagccctgga ggaggctgtg cagatgatga cagaggccgt agaagacctg
     4921 acgacgaccc tcaacgaagc agccagtgct gcaggagtcg ttggcggcat ggtggactct
     4981 atcactcagg ccatcaacca gctagatgaa ggacctatgg gtgacccaga aggctcattc
     5041 gtagattacc agacaaccat ggtgaggaca gccaaggcca ttgctgtcac tgttcaggag
     5101 atggtaacca agtcaaacac cagccctgaa gagctaggcc ctcttgccaa ccagctgacc
     5161 agtgactatg gccgactggc ctcacaagcc aagcctgcag ctgtggctgc tgaaaatgaa
     5221 gagataggcg ctcacatcaa acaccgagta caggagctgg gccatggctg ctctgctctg
     5281 gtcaccaagg caggtgcctt gcagtgtagc cccagtgatg tctacaccaa gaaggagctc
     5341 atagagtgtg cccgcagagt gtcagagaag gtctcccatg ttctggctgc actccaggct
     5401 gggaatcgtg gtacccaggc ctgcattaca gcagccagtg ctgtgtctgg tatcattgct
     5461 gacctcgaca ccaccatcat gttcgctact gctggcacac ttaaccgtga gggtgctgaa
     5521 acttttgctg accaccggga gggtatctta aagacagcaa aggttcttgt ggaggacacc
     5581 aaggtcctag tgcagaatgc agctgggagc caggagaagt tggcacaagc cgcccagtcc
     5641 tccgtggcca ccattacccg cctcgctgat gtggtcaagc tcggtgcagc cagcctagga
     5701 gccgaagacc ctgaaactca ggtggtgctg atcaatgcag taaaggacgt agccaaggcc
     5761 ctgggtgacc tcatcagcgc tacgaaggct gcagcgggca aagttgggga tgaccctgca
     5821 gtgtggcagc tcaagaactc tgccaaggtg atggtgacca atgtgacatc attgctcaag
     5881 acagtgaagg ctgtggaaga tgaggccacc aaaggcacac gggccctaga gaagacctct
     5941 acccctgaag acttcatccg aatgaccaag ggtattacta tggcaacagc caaagccgtt
     6001 gctgctggca attcctgtcg acaggaagat gtcattgcca cagccaatct gagccgacgg
     6061 gctattgcgg acatgcttcg ggcttgcaag gaagcagctt tccacccaga agtggcgcct
     6121 gatgtacggc tccgagccct gcactatggc cgggagtgtg ccaatggtta cctggaactg
     6181 ctggaccacg tgctgctgac ccttcagaag ccaaacccag acctgaagca gcagctaact
     6241 ggacactcaa agcgggttgc tggctctgtg actgagctca tccaggctgc tgaagccatg
     6301 aaaggaacag agtgggtgga cccagaggac cctactgtca ttgctgagaa tgagctcttg
     6361 ggagccgcag ccgccatcga ggctgcagcc aagaagctgg agcagctgaa gcccagggcc
     6421 aaacccaagg aggcggatga gtccttgaac tttgaggaac aaatcctaga agctgccaag
     6481 tccatcgctg cagccaccag tgcactggta aaggctgcgt cagcagccca gagggagctg
     6541 gtggctcaag gaaaggtggg cgccattcca gccaatgcac tggatgatgg gcagtggtcg
     6601 cagggcctca tttccgctgc ccgtatggtg gctgcggcca ccaacaatct gtgtgaagca
     6661 gctaacgcag ctgtccaggg ccatgctagc caggagaaac tcatttcctc agccaagcaa
     6721 gtagctgcct ccacagctca gctcctggta gcttgcaagg tcaaagcaga tcaggactct
     6781 gaggcaatga aacggctcca ggctgctggc aatgcagtga agagggcctc agataacctg
     6841 gtaaaggcgg cccagaaagc tgcagccttc gaagaccagg agaatgagac ggtggtggtg
     6901 aaggagaaaa tggttggggg cattgcccag attatcgcag cacaggaaga gatgcttcgg
     6961 aaggaacgag agctggaaga ggctcggaaa aagctcgccc agatccggca gcagcagtac
     7021 aagttcttgc cttcagagct tcgagacgag cactaaaaaa gccctgtgta tttaatgcag
     7081 acccagccca gagactgtgc ctgccactac caaagccttc tgggctgctc aggacccaag
     7141 ctgcccaacc ccagcactcc ccca
//
