LOCUS       sequence-89122-e        2130 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Microtubules, Excitation: 549, Emission: 565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Shaner NC, Lin MZ, McKeown MR, Steinbach PA, Hazelwood KL, Davidson 
            MW, Tsien RY
  TITLE     Improving the photostability of bright monomeric orange and red 
            fluorescent proteins.
  JOURNAL   Nat Methods. 2008 Jun;5(6):545-51. doi: 10.1038/nmeth.1209. Epub 
            2008 May 4.
  PUBMED    18454154
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1209"; journalName: "Nat Methods"; date: "2008-06"; 
            volume: "5"; issue: "6"; pages: "545-51"
COMMENT     Sequence Label: mOrange2-Tubulin-6
FEATURES             Location/Qualifiers
     source          1..2130
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             35..742
                     /codon_start=1
                     /product="photostable monomeric orange derivative of DsRed 
                     fluorescent protein (Shaner et al., 2008)"
                     /label=mOrange2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPHFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GKIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg aggagaataa
       61 catggccatc atcaaggagt tcatgcgctt caaggtgcgc atggagggct ccgtgaacgg
      121 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggct ttcagaccgc
      181 taagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca
      241 tttcacctac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttcaa
      301 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aactacgagg acggcggcgt
      361 ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct
      421 gcgcggcacc aacttcccct ccgacggccc cgtgatgcag aagaagacca tgggctggga
      481 ggcctcctcc gagcggatgt accccgagga cggtgccctg aagggcaaga tcaagatgag
      541 gctgaagctg aaggacggcg gccactacac ctccgaggtc aagaccacct acaaggccaa
      601 gaagcccgtg cagctgcccg gcgcctacat cgtcgacatc aagttggaca tcacctccca
      661 caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg
      721 cggcatggac gagctgtaca agtccggact cagatctcga gtgcgtgagt gcatctccat
      781 ccacgttggc caggctggtg tccagattgg caatgcctgc tgggagctct actgcctgga
      841 acacggcatc cagcccgatg gccagatgcc aagtgacaag accattgggg gaggagatga
      901 ctccttcaac accttcttca gtgagacggg cgctggcaag cacgtgcccc gggctgtgtt
      961 tgtagacttg gaacccacag tcattgatga agttcgcact ggcacctacc gccagctctt
     1021 ccaccctgag cagctcatca caggcaagga agatgctgcc aataactatg cccgagggca
     1081 ctacaccatt ggcaaggaga tcattgacct tgtgttggac cgaattcgca agctggctga
     1141 ccagtgcacc ggtcttcagg gcttcttggt tttccacagc tttggtgggg gaactggttc
     1201 tgggttcacc tccctgctca tggaacgtct ctcagttgat tatggcaaga agtccaagct
     1261 ggagttctcc atttacccag caccccaggt ttccacagct gtagttgagc cctacaactc
     1321 catcctcacc acccacacca ccctggagca ctctgattgt gccttcatgg tagacaatga
     1381 ggccatctat gacatctgtc gtagaaacct cgatatcgag cgcccaacct acactaacct
     1441 taaccgcctt attagccaga ttgtgtcctc catcactgct tccctgagat ttgatggagc
     1501 cctgaatgtt gacctgacag aattccagac caacctggtg ccctaccccc gcatccactt
     1561 ccctctggcc acatatgccc ctgtcatctc tgctgagaaa gcctaccatg aacagctttc
     1621 tgtagcagag atcaccaatg cttgctttga gccagccaac cagatggtga aatgtgaccc
     1681 tcgccatggt aaatacatgg cttgctgcct gttgtaccgt ggtgacgtgg ttcccaaaga
     1741 tgtcaatgct gccattgcca ccatcaaaac caagcgcagc atccagtttg tggattggtg
     1801 ccccactggc ttcaaggttg gcatcaacta ccagcctccc actgtggtgc ctggtggaga
     1861 cctggccaag gtacagagag ctgtgtgcat gctgagcaac accacagcca ttgctgaggc
     1921 ctgggctcgc ctggaccaca agtttgacct gatgtatgcc aagcgtgcct ttgttcactg
     1981 gtacgtgggt gaggggatgg aggaaggcga gttttcagag gcccgtgaag atatggctgc
     2041 ccttgagaag gattatgagg aggttggtgt ggattctgtt gaaggagagg gtgaggaaga
     2101 aggagaggaa tactaaggat ccaccggatc
//
