LOCUS       sequence-89150-e        1236 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 555, Emission: 584.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1236)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 1236)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mTagRFPet-H2B-6
FEATURES             Location/Qualifiers
     source          1..1236
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             66..443
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      456..465
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             462..1193
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
ORIGIN
        1 ctagcgctac cggactcaga tctcgagctc aagcttcgaa ttctgcagtc gacggtaccg
       61 ccaccatgcc agagccagcg aagtctgctc ccgccccgaa aaagggctcc aagaaggcgg
      121 tgactaaggc gcagaagaaa ggcggcaaga agcgcaagcg cagccgcaag gagagctatt
      181 ccatctatgt gtacaaggtt ctgaagcagg tccaccctga caccggcatt tcgtccaagg
      241 ccatgggcat catgaattcg tttgtgaacg acattttcga gcgcatcgca ggtgaggctt
      301 cccgcctggc gcattacaac aagcgctcga ccatcacctc cagggagatc cagacggccg
      361 tgcgcctgct gctgcctggg gagttggcca agcacgccgt gtccgagggt actaaggcca
      421 tcaccaagta caccagcgct aaggatccac cggtcgccac catggtgtct aagggcgaag
      481 agctgattaa ggagaacatg cacatgaagc tgtacatgga gggcaccgtg aacaaccacc
      541 acttcaagtg cacatccgag ggcgaaggca agccctacga gggcacccag accatgagaa
      601 tcaaggtggt cgagggcggc cctctcccct tcgccttcga catcctggct accagcttca
      661 tgtacggcag cagaaccttc atcaaccaca cccagggcat ccccgatttc tttaagcagt
      721 ccttccctga gggcttcaca tgggagagag tcaccacata cgaagacggg ggcgtgctga
      781 ccgctaccca ggacaccagc ctccaggacg gctgcctcat ctacaacgtc aagatcagag
      841 gggtgaactt cccatccaac ggccctgtga tgcagaagaa aacactcggc tgggaggcca
      901 acaccgagat gctgtacccc gctgacggcg gcctggaagg cagaagcgac atggccctga
      961 agctcgtggg cgggggccac ctgatctgca acttcaagac cacatacaga tccaagaaac
     1021 ccgctaagaa cctcaagatg cccggcgtct actatgtgga ccacagactg gaaagaatca
     1081 aggaggccga caaagagacc tacgtcgagc agcacgaggt ggctgtggcc agatactgcg
     1141 acctccctag caaactgggg cacaaactta atggcatgga cgagctgtac aagtaagcgg
     1201 ccgcgactct agatcataat cagccatacc acattg
//
