LOCUS       sequence-89155-e        1491 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Clathrin Vesicles, Excitation: 555, Emission: 584.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1491)
  AUTHORS   Shaner NC, Lin MZ, McKeown MR, Steinbach PA, Hazelwood KL, Davidson 
            MW, Tsien RY
  TITLE     Improving the photostability of bright monomeric orange and red 
            fluorescent proteins.
  JOURNAL   Nat Methods. 2008 Jun;5(6):545-51. doi: 10.1038/nmeth.1209. Epub 
            2008 May 4.
  PUBMED    18454154
REFERENCE   2  (bases 1 to 1491)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1209"; journalName: "Nat Methods"; date: "2008-06"; 
            volume: "5"; issue: "6"; pages: "545-51"
COMMENT     Sequence Label: mTagRFP-T-Clathrin-15
FEATURES             Location/Qualifiers
     source          1..1491
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             35..766
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtcgc caccatggtg tctaagggcg aagagctgat
       61 taaggagaac atgcacatga agctgtacat ggagggcacc gtgaacaacc accacttcaa
      121 gtgcacatcc gagggcgaag gcaagcccta cgagggcacc cagaccatga gaatcaaggt
      181 ggtcgagggc ggccctctcc ccttcgcctt cgacatcctg gctaccagct tcatgtacgg
      241 cagcagaacc ttcatcaacc acacccaggg catccccgat ttctttaagc agtccttccc
      301 tgagggcttc acatgggaga gagtcaccac atacgaagac gggggcgtgc tgaccgctac
      361 ccaggacacc agcctccagg acggctgcct catctacaac gtcaagatca gaggggtgaa
      421 cttcccatcc aacggccctg tgatgcagaa gaaaacactc ggctgggagg ccaacaccga
      481 gatgctgtac cccgctgacg gcggcctgga aggcagaacc gacatggccc tgaagctcgt
      541 gggcgggggc cacctgatct gcaacttcaa gaccacatac agatccaaga aacccgctaa
      601 gaacctcaag atgcccggcg tctactatgt ggaccacaga ctggaaagaa tcaaggaggc
      661 cgacaaagag acctacgtcg agcagcacga ggtggctgtg gccagatact gcgacctccc
      721 tagcaaactg gggcacaaac ttaatggcat ggacgagctg tacaagtccg gactcagatc
      781 tcgagctcaa gcttcgaatt ctgcagtcga catggctgat gactttggct tcttctcgtc
      841 gtcggagagt ggtgccccgg aggcggcgga ggaggacccg gcggccgcct tcctggccca
      901 gcaggagagc gagattgcag gcatagagaa cgacgagggc ttcggggcac ctgccggcag
      961 ccatgcggcc cccgcacagc cgggccccac gagtggggct ggttctgagg acatggggac
     1021 cacagtcaat ggagatgtgt ttcaggaggc caacggtcct gctgatggct acgcagccat
     1081 tgcccaggct gacaggctga cccaggagcc tgagagcatc cgcaagtggc gagaggagca
     1141 gaggaaacgg ctgcaagagc tggatgctgc atctaaggtc acggaacagg aatggcggga
     1201 gaaggccaag aaggacctgg aggagtggaa ccagcgccag agtgaacaag tagagaagaa
     1261 caagatcaac aaccgggcat ccgaggaggc tttcgtgaag gaatccaagg aggagacccc
     1321 aggcacagag tgggagaagg tggcccagct atgtgacttc aaccccaaga gcagcaagca
     1381 gtgcaaagat gtgtcccgcc tgcgctcggt gctcatgtcc ctgaagcaga cgccactgtc
     1441 ccgctaggga tccaccggat ctagataact gatcataatc agccatacca c
//
