LOCUS       sequence-89165-e        1775 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleoli, Excitation: 555, Emission: 584.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Shaner NC, Lin MZ, McKeown MR, Steinbach PA, Hazelwood KL, Davidson 
            MW, Tsien RY
  TITLE     Improving the photostability of bright monomeric orange and red 
            fluorescent proteins.
  JOURNAL   Nat Methods. 2008 Jun;5(6):545-51. doi: 10.1038/nmeth.1209. Epub 
            2008 May 4.
  PUBMED    18454154
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1209"; journalName: "Nat Methods"; date: "2008-06"; 
            volume: "5"; issue: "6"; pages: "545-51"
COMMENT     Sequence Label: mTagRFP-T-Fibrillarin-7
FEATURES             Location/Qualifiers
     source          1..1775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             35..766
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
     CDS             complement(1562..1573)
                     /codon_start=1
                     /gene="cspA"
                     /product="translation enhancing element for E. coli (Qing 
                     et al., 2004)"
                     /label=TEE
                     /translation="NHKV"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtcgc caccatggtg tctaagggcg aagagctgat
       61 taaggagaac atgcacatga agctgtacat ggagggcacc gtgaacaacc accacttcaa
      121 gtgcacatcc gagggcgaag gcaagcccta cgagggcacc cagaccatga gaatcaaggt
      181 ggtcgagggc ggccctctcc ccttcgcctt cgacatcctg gctaccagct tcatgtacgg
      241 cagcagaacc ttcatcaacc acacccaggg catccccgat ttctttaagc agtccttccc
      301 tgagggcttc acatgggaga gagtcaccac atacgaagac gggggcgtgc tgaccgctac
      361 ccaggacacc agcctccagg acggctgcct catctacaac gtcaagatca gaggggtgaa
      421 cttcccatcc aacggccctg tgatgcagaa gaaaacactc ggctgggagg ccaacaccga
      481 gatgctgtac cccgctgacg gcggcctgga aggcagaacc gacatggccc tgaagctcgt
      541 gggcgggggc cacctgatct gcaacttcaa gaccacatac agatccaaga aacccgctaa
      601 gaacctcaag atgcccggcg tctactatgt ggaccacaga ctggaaagaa tcaaggaggc
      661 cgacaaagag acctacgtcg agcagcacga ggtggctgtg gccagatact gcgacctccc
      721 tagcaaactg gggcacaaac ttaatggcat ggacgagctg tacaagtccg gactcagatc
      781 tcgagccatg aagccaggat tcagtccccg tgggggtggc tttggcggcc gagggggctt
      841 tggtgaccgt ggtggtcgtg gaggccgagg gggctttggc gggggccgag gccaaggcgg
      901 aggctttaga ggtcgtggac gaggaggagg tggaggcggc ggcggcggtg gaggaggagg
      961 aagaggtggt ggaggcttcc attttggtgg caaccggggt cgtggtcggg gaggaaaaag
     1021 aggaaaccag tcggggaaga atgtgatggt ggagccgcat cggcatgagg gtgtcttcat
     1081 ttgtcgggga aaggaagatg cactggtcac caagaacctg gtccctgggg aatcagttta
     1141 tggagagaag agagtctcga tttcggaagg agatgacaaa attgagtacc gagcctggaa
     1201 ccccttccgc tccaagcttg cagcagcaat cctgggtggt gtggaccaga tccacatcaa
     1261 accgggggct aaggttctct acctcggggc tgcctcgggc accacggtct cccatgtctc
     1321 tgacatcgtt ggtccggatg gtctagtcta tgcagtcgag ttctcccacc gctctggccg
     1381 tgacctcatt aacttggcca agaagaggac caacatcatt cctgtgatcg aggatgctcg
     1441 acacccacac aaataccgca tgctcatcgc aatggtggat gtgatctttg ctgatgtggc
     1501 ccagccagac cagacccgga ttgtggccct gaatgcccac accttcctgc gtaatggagg
     1561 acactttgtg atttccatta aggccaactg cattgactcc acagcctcag ccgaggccgt
     1621 gtttgcctcc gaagtgaaaa agatgcaaca ggagaacatg aagccgcagg agcagttgac
     1681 ccttgagcca tatgaaagag accatgccgt ggtcgtggga gtgtacaggc caccccccaa
     1741 ggtgaagaac tgaggatcca ccggatctag ataac
//
