LOCUS       sequence-89183-e        1207 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 553, Emission: 574.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1207)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 1207)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: TurboRFP-H2B-6
FEATURES             Location/Qualifiers
     source          1..1207
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             77..454
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     CDS             473..1168
                     /codon_start=1
                     /product="red fluorescent protein from Entacmaea 
                     quadricolor"
                     /label=TurboRFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
ORIGIN
        1 cgtcagatcc gctagcgcta ccggactcag atctcgagct caagcttcga attctgcagt
       61 cgacggtacc gccaccatgc cagagccagc gaagtctgct cccgccccga aaaagggctc
      121 caagaaggcg gtgactaagg cgcagaagaa aggcggcaag aagcgcaagc gcagccgcaa
      181 ggagagctat tccatctatg tgtacaaggt tctgaagcag gtccaccctg acaccggcat
      241 ttcgtccaag gccatgggca tcatgaattc gtttgtgaac gacattttcg agcgcatcgc
      301 aggtgaggct tcccgcctgg cgcattacaa caagcgctcg accatcacct ccagggagat
      361 ccagacggcc gtgcgcctgc tgctgcctgg ggagttggcc aagcacgccg tgtccgaggg
      421 tactaaggcc atcaccaagt acaccagcgc taaggatcca ccggtcgcca ccatgagcga
      481 gctgatcaag gagaacatgc acatgaagct gtacatggag ggcaccgtga acaaccacca
      541 cttcaagtgc acatccgagg gcgaaggcaa gccctacgag ggcacccaga ccatgaagat
      601 caaggtggtc gagggcggcc ctctcccctt cgccttcgac atcctggcta ccagcttcat
      661 gtacggcagc aaagccttca tcaaccacac ccagggcatc cccgacttct ttaagcagtc
      721 cttccctgag ggcttcacat gggagagaat caccacatac gaagacgggg gcgtgctgac
      781 cgctacccag gacaccagct tccagaacgg ctgcatcatc tacaacgtca agatcaacgg
      841 ggtgaacttc ccatccaacg gccctgtgat gcagaagaaa acacgcggct gggaggccaa
      901 caccgagatg ctgtaccccg ctgacggcgg cctgagaggc cacagccaga tggccctgaa
      961 gctcgtgggc gggggctacc tgcactgctc cttcaagacc acatacagat ccaagaaacc
     1021 cgctaagaac ctcaagatgc ccggcttcca cttcgtggac cacagactgg aaagaatcaa
     1081 ggaggccgac aaagagacct acgtcgagca gcacgagatg gctgtggcca agtactgcga
     1141 cctccctagc aaactggggc acagatgatg cggccgcgac tctagatcat aatcagccat
     1201 accacat
//
