LOCUS       sequence-89229-e        2490 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nuclear - Nucleolus, Excitation: 554, Emission: 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2490)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 2490)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdTomato-Fibrillarin-7
FEATURES             Location/Qualifiers
     source          1..2490
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      23..32
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             29..1456
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     CDS             complement(2252..2263)
                     /codon_start=1
                     /gene="cspA"
                     /product="translation enhancing element for E. coli (Qing 
                     et al., 2004)"
                     /label=TEE
                     /translation="NHKV"
ORIGIN
        1 agatccgcta gcgctaccgg tcgccaccat ggtgagcaag ggcgaggagg tcatcaaaga
       61 gttcatgcgc ttcaaggtgc gcatggaggg ctccatgaac ggccacgagt tcgagatcga
      121 gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa
      181 gggcggcccc ctgcccttcg cctgggacat cctgtccccc cagttcatgt acggctccaa
      241 ggcgtacgtg aagcaccccg ccgacatccc cgattacaag aagctgtcct tccccgaggg
      301 cttcaagtgg gagcgcgtga tgaacttcga ggacggcggt ctggtgaccg tgacccagga
      361 ctcctccctg caggacggca cgctgatcta caaggtgaag atgcgcggca ccaacttccc
      421 ccccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcca ccgagcgcct
      481 gtacccccgc gacggcgtgc tgaagggcga gatccaccag gccctgaagc tgaaggacgg
      541 cggccactac ctggtggagt tcaagaccat ctacatggcc aagaagcccg tgcaactgcc
      601 cggctactac tacgtggaca ccaagctgga catcacctcc cacaacgagg actacaccat
      661 cgtggaacag tacgagcgct ccgagggccg ccaccacctg ttcctggggc atggcaccgg
      721 cagcaccggc agcggcagct ccggcaccgc ctcctccgag gacaacaaca tggccgtcat
      781 caaagagttc atgcgcttca aggtgcgcat ggagggctcc atgaacggcc acgagttcga
      841 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt
      901 gaccaagggc ggccccctgc ccttcgcctg ggacatcctg tccccccagt tcatgtacgg
      961 ctccaaggcg tacgtgaagc accccgccga catccccgat tacaagaagc tgtccttccc
     1021 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggtctgg tgaccgtgac
     1081 ccaggactcc tccctgcagg acggcacgct gatctacaag gtgaagatgc gcggcaccaa
     1141 cttccccccc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctccaccga
     1201 gcgcctgtac ccccgcgacg gcgtgctgaa gggcgagatc caccaggccc tgaagctgaa
     1261 ggacggcggc cactacctgg tggagttcaa gaccatctac atggccaaga agcccgtgca
     1321 actgcccggc tactactacg tggacaccaa gctggacatc acctcccaca acgaggacta
     1381 caccatcgtg gaacagtacg agcgctccga gggccgccac cacctgttcc tgtacggcat
     1441 ggacgagctg tacaagtccg gactcagatc tcgagccatg aagccaggat tcagtccccg
     1501 tgggggtggc tttggcggcc gagggggctt tggtgaccgt ggtggtcgtg gaggccgagg
     1561 gggctttggc gggggccgag gccaaggcgg aggctttaga ggtcgtggac gaggaggagg
     1621 tggaggcggc ggcggcggtg gaggaggagg aagaggtggt ggaggcttcc attttggtgg
     1681 caaccggggt cgtggtcggg gaggaaaaag aggaaaccag tcggggaaga atgtgatggt
     1741 ggagccgcat cggcatgagg gtgtcttcat ttgtcgggga aaggaagatg cactggtcac
     1801 caagaacctg gtccctgggg aatcagttta tggagagaag agagtctcga tttcggaagg
     1861 agatgacaaa attgagtacc gagcctggaa ccccttccgc tccaagctag cagcagcaat
     1921 cctgggtggt gtggaccaga tccacatcaa accgggggct aaggttctct acctcggggc
     1981 tgcctcgggc accacggtct cccatgtctc tgacatcgtt ggtccggatg gtctagtcta
     2041 tgcagtcgag ttctcccacc gctctggccg tgacctcatt aacttggcca agaagaggac
     2101 caacatcatt cctgtgatcg aggatgctcg acacccacac aaataccgca tgctcatcgc
     2161 aatggtggat gtgatctttg ctgatgtggc ccagccagac cagacccgga ttgtggccct
     2221 gaatgcccac accttcctgc gtaatggagg acactttgtg atttccatta aggccaactg
     2281 cattgactcc acagcctcag ccgaggccgt gtttgcctcc gaagtgaaaa agatgcaaca
     2341 ggagaacatg aagccgcagg agcagttgac ccttgagcca tatgaaagag accatgccgt
     2401 ggtcgtggga gtgtacaggc caccccccaa ggtgaagaac tgaggatcca ccggatctag
     2461 ataactgatc ataatcagcc ataccacatt
//
