LOCUS       sequence-89230-e        1648 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nuclear - Nucleolus, Excitation: 554, Emission: 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1648)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdTomato-FTR-940
FEATURES             Location/Qualifiers
     source          1..1648
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      175..184
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             181..1611
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
ORIGIN
        1 acgtcagatc cgctagcgct accggactca gatctcgagc tcaagcttat gggcatggcg
       61 cgaccacggg gcgcggggcc ctgcagcccc gggttggagc gggctccgcg ccggagcgtc
      121 ggggagctgc gcctgctctt cgaagcgggc ggatctggat cggatccacc ggtcgccacc
      181 atggtgagca agggcgagga ggtcatcaaa gagttcatgc gcttcaaggt gcgcatggag
      241 ggctccatga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
      301 ggcacccaga ccgccaagct gaaggtgacc aagggcggcc ccctgccctt cgcctgggac
      361 atcctgtccc cccagttcat gtacggctcc aaggcgtacg tgaagcaccc cgccgacatc
      421 cccgattaca agaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
      481 gaggacggcg gtctggtgac cgtgacccag gactcctccc tgcaggacgg cacgctgatc
      541 tacaaggtga agatgcgcgg caccaacttc ccccccgacg gccccgtaat gcagaagaag
      601 accatgggct gggaggcctc caccgagcgc ctgtaccccc gcgacggcgt gctgaagggc
      661 gagatccacc aggccctgaa gctgaaggac ggcggccact acctggtgga gttcaagacc
      721 atctacatgg ccaagaagcc cgtgcaactg cccggctact actacgtgga caccaagctg
      781 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgagcg ctccgagggc
      841 cgccaccacc tgttcctggg gcatggcacc ggcagcaccg gcagcggcag ctccggcacc
      901 gcctcctccg aggacaacaa catggccgtc atcaaagagt tcatgcgctt caaggtgcgc
      961 atggagggct ccatgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc
     1021 tacgagggca cccagaccgc caagctgaag gtgaccaagg gcggccccct gcccttcgcc
     1081 tgggacatcc tgtcccccca gttcatgtac ggctccaagg cgtacgtgaa gcaccccgcc
     1141 gacatccccg attacaagaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg
     1201 aacttcgagg acggcggtct ggtgaccgtg acccaggact cctccctgca ggacggcacg
     1261 ctgatctaca aggtgaagat gcgcggcacc aacttccccc ccgacggccc cgtaatgcag
     1321 aagaagacca tgggctggga ggcctccacc gagcgcctgt acccccgcga cggcgtgctg
     1381 aagggcgaga tccaccaggc cctgaagctg aaggacggcg gccactacct ggtggagttc
     1441 aagaccatct acatggccaa gaagcccgtg caactgcccg gctactacta cgtggacacc
     1501 aagctggaca tcacctccca caacgaggac tacaccatcg tggaacagta cgagcgctcc
     1561 gagggccgcc accacctgtt cctgtacggc atggacgagc tgtacaagta agcggccgcg
     1621 actctagatc ataatcagcc ataccaca
//
