LOCUS       sequence-89250-e        2556 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cilium, Excitation: 554, Emission: 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2556)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 2556)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdTomato-MCHR1-N-10
FEATURES             Location/Qualifiers
     source          1..2556
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1084..1093
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1090..2517
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
ORIGIN
        1 atggatctgc aagcctcgtt gctgtccact ggccccaatg ccagcaacat ctccgatggc
       61 caggataatt tcacattggc ggggccacct cctcgcacaa ggagtgtctc ctacatcaac
      121 atcatcatgc cttcagtgtt tggtaccatc tgtctcctgg gcattgtggg aaactccaca
      181 gtcatttttg ccgtggtgaa gaaatccaag ctgcactggt gcagcaacgt ccctgacatc
      241 ttcatcatca acctctctgt ggtggatctg cttttcctgc tgggcatgcc tttcatgatc
      301 caccagctca tgggtaatgg tgtctggcac tttggggaaa ccatgtgcac cctcatcaca
      361 gccatggacg ccaacagtca gttcaccagc acctacatcc tgactgctat ggccattgac
      421 cgctacttgg ccaccgtcca tcccatctcc tccaccaagt tccggaagcc ctccatggcc
      481 accctggtga tctgcctcct gtgggctctc tcgttcatta gcatcactcc tgtgtggctc
      541 tatgccaggc ttatcccctt cccagggggt gctgtgggct gtggcatccg cctaccaaac
      601 ccagatactg atctttactg gttcactctg tatcagtttt tcctggcctt cgcccttccg
      661 tttgtggtca tcactgctgc gtacgtgaaa atactacagc gcatgacgtc ttcggtggcc
      721 ccagcctctc aacgcagcat ccggcttcgg acaaagaggg tgacccgcac agccattgcc
      781 atctgtctgg tcttctttgt gtgctgggcg ccctactacg tgctgcagct gacccagttg
      841 tccatcagcc gcccgaccct cacattcgtc tacctgtaca atgcggctat cagcttgggc
      901 tatgccaaca gctgcctcaa tccctttgtg tacatagtac tctgtgagac ctttcgaaaa
      961 cgcttggtgc tgtcggtgaa gcccgcggcc caggggcagc ttcgcacggt cagcaatgct
     1021 cagacagctg acgaggagag gacagaaagc aaaggcaccg gaggtagcgg ggatccaccg
     1081 gtcgccacca tggtgagcaa gggcgaggag gtcatcaaag agttcatgcg cttcaaggtg
     1141 cgcatggagg gctccatgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc
     1201 ccctacgagg gcacccagac cgccaagctg aaggtgacca agggcggccc cctgcccttc
     1261 gcctgggaca tcctgtcccc ccagttcatg tacggctcca aggcgtacgt gaagcacccc
     1321 gccgacatcc ccgattacaa gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg
     1381 atgaacttcg aggacggcgg tctggtgacc gtgacccagg actcctccct gcaggacggc
     1441 acgctgatct acaaggtgaa gatgcgcggc accaacttcc cccccgacgg ccccgtaatg
     1501 cagaagaaga ccatgggctg ggaggcctcc accgagcgcc tgtacccccg cgacggcgtg
     1561 ctgaagggcg agatccacca ggccctgaag ctgaaggacg gcggccacta cctggtggag
     1621 ttcaagacca tctacatggc caagaagccc gtgcaactgc ccggctacta ctacgtggac
     1681 accaagctgg acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgagcgc
     1741 tccgagggcc gccaccacct gttcctgggg catggcaccg gcagcaccgg cagcggcagc
     1801 tccggcaccg cctcctccga ggacaacaac atggccgtca tcaaagagtt catgcgcttc
     1861 aaggtgcgca tggagggctc catgaacggc cacgagttcg agatcgaggg cgagggcgag
     1921 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg cggccccctg
     1981 cccttcgcct gggacatcct gtccccccag ttcatgtacg gctccaaggc gtacgtgaag
     2041 caccccgccg acatccccga ttacaagaag ctgtccttcc ccgagggctt caagtgggag
     2101 cgcgtgatga acttcgagga cggcggtctg gtgaccgtga cccaggactc ctccctgcag
     2161 gacggcacgc tgatctacaa ggtgaagatg cgcggcacca acttcccccc cgacggcccc
     2221 gtaatgcaga agaagaccat gggctgggag gcctccaccg agcgcctgta cccccgcgac
     2281 ggcgtgctga agggcgagat ccaccaggcc ctgaagctga aggacggcgg ccactacctg
     2341 gtggagttca agaccatcta catggccaag aagcccgtgc aactgcccgg ctactactac
     2401 gtggacacca agctggacat cacctcccac aacgaggact acaccatcgt ggaacagtac
     2461 gagcgctccg agggccgcca ccacctgttc ctgtacggca tggacgagct gtacaaggcg
     2521 gccgcgactc tagatcataa tcagccatac cacatt
//
