LOCUS       sequence-89252-e        7427 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytoskeleton, Excitation: 554, Emission: 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7427)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 7427)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdTomato-MyosinIIA-C-18
FEATURES             Location/Qualifiers
     source          1..7427
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             35..1462
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
ORIGIN
        1 accgctcgag ccgctagcgc taccggtcgc caccatggtg agcaagggcg aggaggtcat
       61 caaagagttc atgcgcttca aggtgcgcat ggagggctcc atgaacggcc acgagttcga
      121 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt
      181 gaccaagggc ggccccctgc ccttcgcctg ggacatcctg tccccccagt tcatgtacgg
      241 ctccaaggcg tacgtgaagc accccgccga catccccgat tacaagaagc tgtccttccc
      301 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggtctgg tgaccgtgac
      361 ccaggactcc tccctgcagg acggcacgct gatctacaag gtgaagatgc gcggcaccaa
      421 cttccccccc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctccaccga
      481 gcgcctgtac ccccgcgacg gcgtgctgaa gggcgagatc caccaggccc tgaagctgaa
      541 ggacggcggc cactacctgg tggagttcaa gaccatctac atggccaaga agcccgtgca
      601 actgcccggc tactactacg tggacaccaa gctggacatc acctcccaca acgaggacta
      661 caccatcgtg gaacagtacg agcgctccga gggccgccac cacctgttcc tggggcatgg
      721 caccggcagc accggcagcg gcagctccgg caccgcctcc tccgaggaca acaacatggc
      781 cgtcatcaaa gagttcatgc gcttcaaggt gcgcatggag ggctccatga acggccacga
      841 gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct
      901 gaaggtgacc aagggcggcc ccctgccctt cgcctgggac atcctgtccc cccagttcat
      961 gtacggctcc aaggcgtacg tgaagcaccc cgccgacatc cccgattaca agaagctgtc
     1021 cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gtctggtgac
     1081 cgtgacccag gactcctccc tgcaggacgg cacgctgatc tacaaggtga agatgcgcgg
     1141 caccaacttc ccccccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc
     1201 caccgagcgc ctgtaccccc gcgacggcgt gctgaagggc gagatccacc aggccctgaa
     1261 gctgaaggac ggcggccact acctggtgga gttcaagacc atctacatgg ccaagaagcc
     1321 cgtgcaactg cccggctact actacgtgga caccaagctg gacatcacct cccacaacga
     1381 ggactacacc atcgtggaac agtacgagcg ctccgagggc cgccaccacc tgttcctgta
     1441 cggcatggac gagctgtaca agtccggact cagatctggc agcggtggag gcagcgcatc
     1501 cggcggaagc ggaagcgctc agcaggctgc agacaagtac ctctatgtgg ataaaaactt
     1561 catcaataac ccgctggccc aagctgactg ggctgccaag aagttggtat gggtgccttc
     1621 cagcaagaat ggctttgaac cagccagcct caaggaggag gtgggagaag aggccattgt
     1681 agagctggta gagaatggga agaaggtgaa ggtgaacaag gacgacatcc agaagatgaa
     1741 cccacccaag ttctccaagg tggaggacat ggcagagctc acgtgcctca acgaagcttc
     1801 ggtgctgcac aacctcaagg agcgatacta ctcagggctt atctacacct attcaggcct
     1861 gttctgtgtg gtcatcaacc cttataagaa cctgcccatc tactcagagg agatcgtgga
     1921 gatgtacaag ggcaagaaga ggcacgagat gccaccccac atctacgcca tcacagatac
     1981 tgcctaccgg agcatgatgc aggaccggga agatcagtcc atcctgtgca cgggggagtc
     2041 tggagcaggg aagacagaga acaccaagaa agtcatccag tacctggcac atgtggcctc
     2101 ctcacacaag agcaagaagg accaggggga gttggagcgg cagctgctac aggccaaccc
     2161 tatcctagag gcctttggaa acgccaagac ggtgaagaat gacaactcct ctcgattcgg
     2221 taaattcatt cgtatcaact ttgatgtcaa tggctacatt gttggtgcca acattgagac
     2281 ttatcttctg gagaaatctc gtgctatccg ccaagccaaa gaggagcgga ccttccacat
     2341 cttctactac ctgctgtctg gggccggaga acacctgaag actgatctcc tgttggagcc
     2401 atacaacaaa taccgcttcc tgtccaacgg gcacgtcacc atccctgggc agcaggacaa
     2461 ggacatgttc caggagacaa tggaggccat gagaattatg ggtatcccag aggatgagca
     2521 gatgggcttg ctgcgggtca tctctggggt ccttcagctt ggcaacattg ccttcaagaa
     2581 ggagcggaac actgaccagg cgtccatgcc ggacaacaca gctgctcaaa aggtgtccca
     2641 cctcctgggg atcaatgtga ccgacttcac cagaggcatc ctcaccccac gcatcaaggt
     2701 gggcagagac tatgtgcaga aggcgcagac taaagagcag gctgactttg ccattgaggc
     2761 cttggccaag gctacctatg agcggatgtt ccgctggctg gtgcttcgca tcaacaaagc
     2821 tctggacaag accaagaggc agggcgcctc atttatcggg atactggaca tcgctggctt
     2881 tgaaatcttt gatctgaact ccttcgagca gctgtgcatc aactacacca acgagaagct
     2941 gcagcagctg ttcaaccaca ccatgttcat cctggagcag gaggagtacc agcgagaggg
     3001 catcgagtgg aacttcatcg acttcggcct ggacctgcag ccctgcatcg acctcattga
     3061 gaagccggcg ggtcccccag gcatcctggc cctgctagat gaggagtgct ggtttcctaa
     3121 ggccactgac aagagcttcg tggagaaggt ggtgcaggag cagggcaccc accccaagtt
     3181 ccagaagccc aagcaactga aggacaaggc tgatttctgc attatccact atgccggcaa
     3241 ggtggactat aaagctgacg agtggctgat gaagaacatg gaccccttga acgacaacat
     3301 cgccacgctg cttcaccagt cctcagacaa gtttgtctct gagctgtgga aggatgtgga
     3361 tcggatcatt ggcttggacc aagtggctgg aatgtccgag acagcactac ctggtgcctt
     3421 caagacccgg aagggcatgt tccgtactgt cggacagctg tacaaggagc agctggccaa
     3481 gctcatggcc acgttgagga ataccaaccc caacttcgtg cgctgcatca ttcccaacca
     3541 tgagaagaag gccggcaaac tggacccgca cttggtgctg gaccagctgc gctgcaatgg
     3601 cgtccttgag ggcatccgga tctgccgcca gggctttccc aacagggtgg tcttccagga
     3661 gttccggcag aggtatgaga tcctcacccc caactccatc ccgaagggct tcatggatgg
     3721 caagcaagcg tgtgtgctca tgatcaaagc cttggagctt gacagcaacc tgtaccgcat
     3781 cggccagagc aaagtgttct tccgggcagg agtgctcgcc cacctggagg aagagcggga
     3841 cctgaagatc accgatgtca tcattggctt ccaggcctgc tgcaggggct acctggccag
     3901 gaaggccttt gccaagaggc agcaacagct gaccgccatg aaggtcctac agaggaactg
     3961 tgctgcgtac ctcaggctgc gcaactggca gtggtggagg ctcttcacca aggtcaagcc
     4021 cctgttgaac tcaataagac atgaggatga gctgttagcc aaggaggcgg aactgacaaa
     4081 ggttcgagag aaacatctgg ctgcagagaa caggctgaca gagatggaga cgatgcagtc
     4141 tcagctcatg gcagagaagc tgcagcttca ggagcagctg caggcggaga cagagctgtg
     4201 tgccgaggct gaggagctcc gggcccgtct gacagcgaag aagcaggagc tggaggagat
     4261 atgccatgac ctggaggcca gggtggagga ggaggaggag cgctgccagt acctgcaggc
     4321 cgagaagaag aagatgcagc agaacatcca ggaacttgag gagcagttgg aggaggagga
     4381 gagcgcccgg cagaagctgc agcttgagaa ggtgaccacc gaggccaagc tgaagaaact
     4441 ggaggaggac cagatcatca tggaggacca gaactgcaaa ctggccaagg agaagaaact
     4501 gctggaagac agagtagctg agttcactac caacctcatg gaagaggagg agaagtccaa
     4561 gagcctggcc aagctcaaga acaagcacga ggcaatgatc accgacctgg aagagcgcct
     4621 ccgtagggag gagaagcaga ggcaggagtt ggagaagacc cgtcgcaagc tggagggaga
     4681 ctccacagac ctcagtgacc agattgctga gctccaggcg cagatagcag agctcaagat
     4741 gcagctggcc aagaaggagg aggagttgca ggctgccttg gccagagtgg aagaagaagc
     4801 tgctcagaag aatatggccc tgaagaagat ccgagaactg gaaactcaga tatctgagct
     4861 ccaggaggac ctggagtcgg agcgagcctc caggaataaa gccgagaagc agaaacggga
     4921 tctgggagag gagctggagg cgctgaagac agagctggag gacacgctgg actccacggc
     4981 tgcccagcag gagctgaggt cgaagcgtga gcaggaggtg agcatcctga agaagactct
     5041 ggaggacgag gccaagaccc atgaggccca gatccaggag atgaggcaga agcactcaca
     5101 ggctgtggag gagctggcag atcagttgga gcagacgaag cgggtaaaag ctacccttga
     5161 gaaggcgaag cagaccctgg agaatgagcg gggagagctg gccaatgagg tgaaggccct
     5221 gctgcaaggc aagggcgact cagagcacaa gcgcaagaag gtggaggcgc agctgcaaga
     5281 actgcaggtc aagttcagcg agggagagcg cgtgcgaacc gaactggccg acaaggtcac
     5341 caagctgcag gttgaactgg acagtgtgac cggtctcctt agccagtctg acagcaagtc
     5401 cagcaagctt acgaaggact tctctgcgct ggagtcccag cttcaggaca cacaggagtt
     5461 gctccaggag gagaaccggc agaagctgag cctgagcacc aagctcaagc agatggagga
     5521 tgagaaaaac tccttcaggg agcagctgga ggaggaggag gaggccaagc gcaacttgga
     5581 gaagcagatc gccacgctcc atgcccaggt gaccgacatg aagaagaaga tggaggacgg
     5641 tgtagggtgc ctggagactg cagaggaggc gaagcggagg cttcagaagg acttggaagg
     5701 cctgagccag cggcttgagg agaaggtggc tgcctacgat aagctggaga agaccaagac
     5761 acggctgcag caggagctgg acgacctgct ggttgacctg gaccaccagc ggcagagcgt
     5821 ctccaacctg gaaaagaagc agaagaagtt cgaccagctc ctagccgagg agaagaccat
     5881 ctcggccaag tatgcagagg agcgtgaccg agctgaggct gaggcccgtg agaaggagac
     5941 aaaggcgcta tcactggccc gggcgcttga ggaggccatg gagcagaagg cagagctgga
     6001 gcggctcaac aagcagttcc gcacggagat ggaggacctc atgagctcca aggatgacgt
     6061 gggcaagagt gtccacgagc tggagaagtc caagcgggcc ttggagcagc aggtggagga
     6121 gatgaagacc cagctggagg agctggagga tgagctgcag gccacggagg atgccaagct
     6181 ccgcctggag gtgaacctgc aggccatgaa ggcccagttt gagcgggatc tgcagggccg
     6241 ggatgaacag agcgaggaga agaagaagca gctggtcaga caggtgcggg agatggaggc
     6301 ggagctggag gatgagagga agcagcgctc catggccatg gccgcacgca agaaactgga
     6361 gatggatctg aaggacctgg aggcacacat tgacacagcc aataagaacc gggaagaggc
     6421 catcaaacag ctgcggaagc ttcaggccca gatgaaggac tgcatgcggg agctggacga
     6481 cacgcgcgcc tcccgggagg agatcctggc gcaggccaag gagaatgaga agaagctgaa
     6541 gagcatggag gccgagatga ttcagctgca ggaggaactg gcagctgctg agcgtgctaa
     6601 gcgtcaggcc caacaggaac gggacgagct ggctgatgag atcgccaaca gcagtggcaa
     6661 aggggcccta gcattagagg agaagcggcg actggaggcc cgcattgccc tgctggagga
     6721 ggagctggag gaggaacagg gcaacacgga gctgatcaac gatcggctga agaaggccaa
     6781 cctgcagatc gaccaaataa acaccgacct gaacctggaa cgcagccacg cacagaagaa
     6841 tgagaatgcg cgacagcagc tggaacgcca gaacaaggag ctcaaggcca agctgcagga
     6901 aatggagagt gctgtcaagt ccaaatacaa ggcctccatc gcggccttgg aggccaaaat
     6961 tgcacagctg gaggaacagc tggacaacga gaccaaggag cgccaggcag cctccaagca
     7021 ggtgcgccgg acggagaaga agctgaagga cgtgctgctg caggtggagg acgagcggag
     7081 gaacgcggaa cagttcaagg accaggctga caaggcgtcc acccgcctga agcagcttaa
     7141 acggcagcta gaggaggctg aagaggaggc ccagcgggcc aatgcctcac gccggaagct
     7201 gcagcgtgag ctggaagatg ccacagagac cgctgatgct atgaaccgcg aggtcagctc
     7261 cctgaagaac aaactgaggc gtggggacct gccatttgtc gtgactcgcc gaattgttcg
     7321 gaaaggcact ggcgactgct cagacgagga ggtcgacggt aaagcagatg gggccgatgc
     7381 caaggcagct gaataggaat tctgcagtcg acggtaccgc gggcccg
//
