LOCUS       sequence-89261-e        2675 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Mitochondria, Excitation: 554, Emission: 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2675)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdTomato-PDHA1-N-10
FEATURES             Location/Qualifiers
     source          1..2675
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1213..1222
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1219..2649
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
ORIGIN
        1 tccgctagca ccgccaccat gaggaagatg ctcgccgccg tctcccgcgt gctgtctggc
       61 gcttctcaga agccggcaag cagagtgctg gtagcatccc gtaattttgc aaatgatgct
      121 acatttgaaa ttaagaaatg tgaccttcac cggctggaag aaggccctcc tgtcacaaca
      181 gtgctcacca gggaggatgg gctcaaatac tacaggatga tgcagactgt acgccgaatg
      241 gagttgaaag cagatcagct gtataaacag aaaattattc gtggtttctg tcacttgtgt
      301 gatggtcagg aagcttgctg tgtgggcctg gaggccggca tcaaccccac agaccatctc
      361 atcacagcct accgggctca cggctttact ttcacccggg gcctttccgt ccgagaaatt
      421 ctcgcagagc ttacaggacg aaaaggaggt tgtgctaaag ggaaaggagg atcgatgcac
      481 atgtatgcca agaacttcta cgggggcaat ggcatcgtgg gagcgcaggt gcccctgggc
      541 gctgggattg ctctagcctg taagtataat ggaaaagatg aggtctgcct gactttatat
      601 ggcgatggtg ctgctaacca gggccagata ttcgaagctt acaacatggc agctttgtgg
      661 aaattacctt gtattttcat ctgtgagaat aatcgctatg gaatgggaac gtctgttgag
      721 agagcggcag ccagcactga ttactacaag agaggcgatt tcattcctgg gctgagagtg
      781 gatggaatgg atatcctgtg cgtccgagag gcaacaaggt ttgctgctgc ctattgtaga
      841 tctgggaagg ggcccatcct gatggagctg cagacttacc gttaccacgg acacagtatg
      901 agtgaccctg gagtcagtta ccgtacacga gaagaaattc aggaagtaag aagtaagagt
      961 gaccctatta tgcttctcaa ggacaggatg gtgaacagca atcttgccag tgtggaagaa
     1021 ctaaaggaaa ttgatgtgga agtgaggaag gagattgagg atgctgccca gtttgccacg
     1081 gccgatcctg agccaccttt ggaagagctg ggctaccaca tctactccag cgacccacct
     1141 tttgaagttc gtggtgccaa tcagtggatc aagtttaagt cagtcagtag ctccggaggg
     1201 gatccaccgg tcgccaccat ggtgagcaag ggcgaggagg tcatcaaaga gttcatgcgc
     1261 ttcaaggtgc gcatggaggg ctccatgaac ggccacgagt tcgagatcga gggcgagggc
     1321 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggcggcccc
     1381 ctgcccttcg cctgggacat cctgtccccc cagttcatgt acggctccaa ggcgtacgtg
     1441 aagcaccccg ccgacatccc cgattacaag aagctgtcct tccccgaggg cttcaagtgg
     1501 gagcgcgtga tgaacttcga ggacggcggt ctggtgaccg tgacccagga ctcctccctg
     1561 caggacggca cgctgatcta caaggtgaag atgcgcggca ccaacttccc ccccgacggc
     1621 cccgtaatgc agaagaagac catgggctgg gaggcctcca ccgagcgcct gtacccccgc
     1681 gacggcgtgc tgaagggcga gatccaccag gccctgaagc tgaaggacgg cggccactac
     1741 ctggtggagt tcaagaccat ctacatggcc aagaagcccg tgcaactgcc cggctactac
     1801 tacgtggaca ccaagctgga catcacctcc cacaacgagg actacaccat cgtggaacag
     1861 tacgagcgct ccgagggccg ccaccacctg ttcctggggc atggcaccgg cagcaccggc
     1921 agcggcagct ccggcaccgc ctcctccgag gacaacaaca tggccgtcat caaagagttc
     1981 atgcgcttca aggtgcgcat ggagggctcc atgaacggcc acgagttcga gatcgagggc
     2041 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggc
     2101 ggccccctgc ccttcgcctg ggacatcctg tccccccagt tcatgtacgg ctccaaggcg
     2161 tacgtgaagc accccgccga catccccgat tacaagaagc tgtccttccc cgagggcttc
     2221 aagtgggagc gcgtgatgaa cttcgaggac ggcggtctgg tgaccgtgac ccaggactcc
     2281 tccctgcagg acggcacgct gatctacaag gtgaagatgc gcggcaccaa cttccccccc
     2341 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctccaccga gcgcctgtac
     2401 ccccgcgacg gcgtgctgaa gggcgagatc caccaggccc tgaagctgaa ggacggcggc
     2461 cactacctgg tggagttcaa gaccatctac atggccaaga agcccgtgca actgcccggc
     2521 tactactacg tggacaccaa gctggacatc acctcccaca acgaggacta caccatcgtg
     2581 gaacagtacg agcgctccga gggccgccac cacctgttcc tgtacggcat ggacgagctg
     2641 tacaagtaag cggccgcgac tctagatcat aatca
//
