LOCUS       sequence-89265-e        2173 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Ras GTPase, Excitation: 554, Emission: 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2173)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 2173)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdTomato-Rab11a-7
FEATURES             Location/Qualifiers
     source          1..2173
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      21..30
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             27..1454
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
ORIGIN
        1 atccgctagc gctaccggtc gccaccatgg tgagcaaggg cgaggaggtc atcaaagagt
       61 tcatgcgctt caaggtgcgc atggagggct ccatgaacgg ccacgagttc gagatcgagg
      121 gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg
      181 gcggccccct gcccttcgcc tgggacatcc tgtcccccca gttcatgtac ggctccaagg
      241 cgtacgtgaa gcaccccgcc gacatccccg attacaagaa gctgtccttc cccgagggct
      301 tcaagtggga gcgcgtgatg aacttcgagg acggcggtct ggtgaccgtg acccaggact
      361 cctccctgca ggacggcacg ctgatctaca aggtgaagat gcgcggcacc aacttccccc
      421 ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctccacc gagcgcctgt
      481 acccccgcga cggcgtgctg aagggcgaga tccaccaggc cctgaagctg aaggacggcg
      541 gccactacct ggtggagttc aagaccatct acatggccaa gaagcccgtg caactgcccg
      601 gctactacta cgtggacacc aagctggaca tcacctccca caacgaggac tacaccatcg
      661 tggaacagta cgagcgctcc gagggccgcc accacctgtt cctggggcat ggcaccggca
      721 gcaccggcag cggcagctcc ggcaccgcct cctccgagga caacaacatg gccgtcatca
      781 aagagttcat gcgcttcaag gtgcgcatgg agggctccat gaacggccac gagttcgaga
      841 tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga
      901 ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc cccccagttc atgtacggct
      961 ccaaggcgta cgtgaagcac cccgccgaca tccccgatta caagaagctg tccttccccg
     1021 agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggtctggtg accgtgaccc
     1081 aggactcctc cctgcaggac ggcacgctga tctacaaggt gaagatgcgc ggcaccaact
     1141 tcccccccga cggccccgta atgcagaaga agaccatggg ctgggaggcc tccaccgagc
     1201 gcctgtaccc ccgcgacggc gtgctgaagg gcgagatcca ccaggccctg aagctgaagg
     1261 acggcggcca ctacctggtg gagttcaaga ccatctacat ggccaagaag cccgtgcaac
     1321 tgcccggcta ctactacgtg gacaccaagc tggacatcac ctcccacaac gaggactaca
     1381 ccatcgtgga acagtacgag cgctccgagg gccgccacca cctgttcctg tacggcatgg
     1441 acgagctgta caagtccgga ctcagatctc gagccatggg cacccgcgac gacgagtacg
     1501 actacctctt taaagttgtc cttattggag attctggtgt tggaaagagt aatctcctgt
     1561 ctcgatttac tcgaaatgag tttaatctgg aaagcaagag caccattgga gtagagtttg
     1621 caacaagaag catccaggtt gatggaaaaa caataaaggc acagatatgg gacacagcag
     1681 ggcaagagcg atatcgagct ataacatcag catattatcg tggagctgta ggtgccttat
     1741 tggtttatga cattgctaaa catctcacat atgaaaatgt agagcgatgg ctgaaagaac
     1801 tgagagatca tgctgatagt aacattgtta tcatgcttgt gggcaataag agtgatctac
     1861 gtcatctcag ggcagttcct acagatgaag caagagcttt tgcagaaaag aatggtttgt
     1921 cattcattga aacttcggcc ctagactcta caaatgtaga agctgctttt cagacaattt
     1981 taacagagat ttaccgcatt gtttctcaga agcaaatgtc agacagacgc gaaaatgaca
     2041 tgtctccaag caacaatgtg gttcctattc atgttccacc aaccactgaa aacaagccaa
     2101 aggtgcagtg ctgtcagaac atctaaggat ccaccggatc tagataactg atcataatca
     2161 gccataccac att
//
