LOCUS       sequence-89270-e        6745 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 554, Emission: 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6745)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 6745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdTomato-Tensin1-N-18
FEATURES             Location/Qualifiers
     source          1..6745
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      5277..5286
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5283..6713
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
ORIGIN
        1 cggactcaga tctcgaggcc accatgagtg tgagccggac catggaggac agctgtgagc
       61 tggacctggt gtacgtcaca gagaggatca tcgctgtctc cttccccagc acagccaatg
      121 aggagaactt tcggagcaac ctccgtgagg tggcgcagat gctcaagtcc aaacatggag
      181 gcaactacct gctgttcaac ctctctgagc ggagacctga catcacgaag ctccatgcca
      241 aggtactgga atttggctgg cccgacctcc acaccccagc cctggagaag atatgcagca
      301 tctgtaaggc catggacaca tggctcaatg cagaccctca caatgtcgtt gttctacaca
      361 acaagggaaa ccgaggcagg ataggagttg tcatcgcggc ttacatgcac tacagcaaca
      421 tttctgccag tgcggaccag gctctggatc ggtttgcaat gaagcggttc tatgaggata
      481 agattgtgcc cattggccag ccatcccaaa gaaggtacgt gcattacttc agtggcctgc
      541 tctccggctc catcaaaatg aacaacaagc ccttgtttct gcaccacgtg atcatgcacg
      601 gcatccccaa ctttgagtct aaaggaggat gtcggccatt tctccgcatc taccaggcca
      661 tgcaacctgt gtacacatct ggcatctaca acatcccagg agacagccag actagcgtct
      721 gcatcaccat cgagccagga ctgctcttga agggagacat cttgctgaag tgctaccaca
      781 agaagttccg aagcccagcc cgagacgtca tcttccgtgt gcagttccac acctgtgcca
      841 tccatgacct gggggttgtc tttgggaagg aggaccttga tgatgctttc aaagatgatc
      901 gatttccaga gtatggcaaa gtggagtttg tattttctta tgggccagag aaaattcaag
      961 gcatggagca cctggagaac gggccgagcg tgtctgtgga ctataacacc tccgaccccc
     1021 tcatccgctg ggactcctac gacaacttca gtgggcatcg agatgacggc atggaggagg
     1081 tggtgggaca cacgcagggg ccactagatg ggagcctgta tgctaaggtg aagaagaaag
     1141 actccctgca cggcagcacc ggggctgtta atgccacacg tcctacactg tcggccaccc
     1201 ccaaccacgt ggaacacacg ctttctgtga gcagcgactc gggcaactcc acagcctcca
     1261 ccaagaccga caagaccgac gagcctgtcc ccggggcctc cagtgccact gctgccttga
     1321 gtccccagga gaagcgggag ctggaccgcc tgctgagtgg ctttggctta gagcgagaga
     1381 agcaaggcgc catgtaccac acccagcacc tcaggtcccg cccagcaggg ggctcggctg
     1441 tgccctcctc tggacgccac gttgtcccag cccaggttca tgtcaatggt ggggcgttag
     1501 catctgagcg ggagacagac atcctggacg atgaattgcc aaaccaggat ggtcacagtg
     1561 cgggcagcat gggcacactc tcttctctgg acggggtcac caacaccagt gaggggggct
     1621 acccagaggc cctgtcccca ctgaccaacg gtctggacaa gtcctacccc atggagccta
     1681 tggtcaatgg aggaggctac ccctacgagt ctgccagccg ggcggggcct gcccatgctg
     1741 gccacacggc ccccatgcgg ccctcctact ctgcacagga gggtttagct ggctaccaga
     1801 gggaggggcc ccacccagcc tggccacagc cagtgaccac ctcccactat gcccatgacc
     1861 ccagcggtat gttccgctct caatcctttt cggaagctga accccagctg cccccagctc
     1921 cggtccgagg gggaagcagc cgggaggctg tgcaaagggg actgaactcg tggcagcagc
     1981 agcagcagca gcagcagcag cctcgcccac ctccacgcca gcaggaaaga gcccacttgg
     2041 agagtcttgt agccagcagg cccagccctc agccattggc agagaccccc atccccagtc
     2101 tccctgagtt cccgcgagca gcctcccagc aggagattga acagtccatc gaaacactca
     2161 atatgctgat gctggacctg gagccagcct ccgctgctgc cccactacac aagtcccaga
     2221 gtgtccccgg ggcctggcca ggggcttctc cactctcctc ccagcccctc tctgggtcct
     2281 cccgtcagtc ccatccactg acccagtcca gatatggcta tatccccagt gggcattcgt
     2341 tgggaacccc tgagccagcc ccacgggcct ctctggagtc tgtccctcct ggcaggtctt
     2401 actcacctta tgactatcag ccatgtttgg ctgggcctaa ccaggatttc cattcaaaga
     2461 gcccagcctc ttcctccttg cctgccttcc ttccgaccac ccacagccct ccagggcctc
     2521 agcaaccccc agcctctctc cctggcctca ctgctcagcc tctgctctca ccaaaggaag
     2581 cgacttcaga cccctcccgg actccagagg aggagccatt gaatttagaa gggctggtgg
     2641 cccacagggt agcaggggta caggctcggg agaagcagcc tgcagagccc ccagcccctc
     2701 tgcggaggcg ggcggccagt gatggacagt atgagaacca gtctccagaa gccacatccc
     2761 ctcgtagccc tggggttcgc tcccctgtcc agtgtgtctc cccggagctg gctcttacca
     2821 tcgctctcaa tcctggaggg cggcccaaag agccccattt gcacagctac aaggaggcct
     2881 tcgaggagat ggagggaacc tccccgagca gcccaccacc cagtggggtg cggtcccccc
     2941 cgggtctggc caagacaccc ctgtctgctc tgggcctgaa acctcacaac ccagcggaca
     3001 tcctgttgca ccccacagga gttaccagaa gacggattca gccagaggaa gatgagggga
     3061 aggtggtggt caggctgtca gaagagcccc ggagctatgt ggagtctgtg gcacggacag
     3121 cggtggctgg accccgagct caggactctg agcccaagag ctttagtgct ccagccaccc
     3181 aggcctatgg ccatgagata cccctgagga acgggaccct gggtggctcc tttgtctccc
     3241 ccagccccct ctccaccagc agccccatcc tcagtgctga cagcacttca gtggggagtt
     3301 tcccgtcggg agagagcagt gaccagggtc cccggacgcc cacccagcct ctgttggagt
     3361 ctggcttccg ctcaggcagc ctgggacagc ccagcccgtc tgcccagaga aactaccaga
     3421 gctcttctcc tctcccgact gtgggcagta gctacagcag ccccgactac tcacttcagc
     3481 atttcagctc ctctccagaa agccaggctc gcgctcagtt cagtgtggct ggcgtccaca
     3541 cggtgcctgg gagccctcag gcgcgccaca gaacagtggg caccaacact ccccctagtc
     3601 ctggcttcgg ctggcgggcc atcaatccca gcatggctgc ccccagcagt cccagtttga
     3661 gccatcacca gatgatgggt ccaccaggca ctggcttcca tggtagcact gtctccagcc
     3721 cccagagcag tgcagcgacc accccgggga gccccagcct gtgtcggcac ccagcagggg
     3781 tctaccaggt ttctggcctc cacaacaaag tggccaccac cccggggagt cccagcctgg
     3841 gccggcaccc tggggctcac caaggcaacc tggcctccgg tcttcatagc aatgcaatag
     3901 ccagccctgg aagccccagc ctgggccgtc acctcggagg gtctggatct gtggttcccg
     3961 gcagcccctg cttggaccgg catgtggcct atggtggcta ttctaccccg gaggatcgga
     4021 gacccacact gtcccggcag agcagtgcct ctggctacca ggctccttcc acgccctcct
     4081 tccctgtctc ccctgcctac taccctggcc tgagcagccc tgccacctcc ccgtcaccag
     4141 actccgcagc cttccggcaa gggagcccaa caccagcctt gccagagaag cgaaggatgt
     4201 cagtgggaga ccgggcaggc agcctcccca actatgccac catcaatggg aaggtgtctt
     4261 cgcctgtcgc cagcggcatg tccagtccca gtgggggcag caccgtctcc ttctcccaca
     4321 ctctgcccga cttctccaag tactccatgc cagacaacag cccggagacg cgggctaaag
     4381 tgaagtttgt ccaggacact tctaagtatt ggtacaagcc tgagatttcc agggagcagg
     4441 ccatcgcgct cctcaaggac caggagccgg gggccttcat catccgcgac agtcactcct
     4501 tccgaggcgc gtacgggctg gccatgaagg tgtcttcgcc acctccaacc atcatgcagc
     4561 agaataaaaa aggagacatg acccatgagc tggtcaggca ttttctgata gagactggcc
     4621 ccagaggagt caagctcaag ggctgcccca atgagccaaa cttcggatcg ctgtctgccc
     4681 tggtctacca gcactccatc atcccattgg ccctgccttg caagctggtc attccaaacc
     4741 gagaccccac agatgaatcg aaagatagct ccggccctgc caactcaact gcagacctgc
     4801 tgaaacaagg ggcagcctgc aatgtgctct tcgtcaactc tgtggacatg gagtcactca
     4861 ctgggccaca ggccatctct aaagccacat ctgagacgtt ggctgcagac cccacgccag
     4921 ctgccaccat cgttcacttc aaagtctctg cccagggaat cactctgact gacaaccaga
     4981 gaaagctctt tttcagacgc cactaccctc tcaacactgt caccttctgt gacctggacc
     5041 cacaggaaag aaagtggatg aaaacagagg gtggtgcccc tgctaagctc ttcggcttcg
     5101 tggcccggaa gcagggcagc accacggaca acgcctgcca cctctttgct gagcttgacc
     5161 ccaaccagcc ggcctctgcc atcgtcaact tcgtctccaa ggtcatgctg aatgccggcc
     5221 aaaagagagg tagcgcatcc gggggtgcgt caggcggcag cggatctgga ccggtcgcca
     5281 ccatggtgag caagggcgag gaggtcatca aagagttcat gcgcttcaag gtgcgcatgg
     5341 agggctccat gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
     5401 agggcaccca gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg
     5461 acatcctgtc cccccagttc atgtacggct ccaaggcgta cgtgaagcac cccgccgaca
     5521 tccccgatta caagaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact
     5581 tcgaggacgg cggtctggtg accgtgaccc aggactcctc cctgcaggac ggcacgctga
     5641 tctacaaggt gaagatgcgc ggcaccaact tcccccccga cggccccgta atgcagaaga
     5701 agaccatggg ctgggaggcc tccaccgagc gcctgtaccc ccgcgacggc gtgctgaagg
     5761 gcgagatcca ccaggccctg aagctgaagg acggcggcca ctacctggtg gagttcaaga
     5821 ccatctacat ggccaagaag cccgtgcaac tgcccggcta ctactacgtg gacaccaagc
     5881 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgag cgctccgagg
     5941 gccgccacca cctgttcctg gggcatggca ccggcagcac cggcagcggc agctccggca
     6001 ccgcctcctc cgaggacaac aacatggccg tcatcaaaga gttcatgcgc ttcaaggtgc
     6061 gcatggaggg ctccatgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc
     6121 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggcggcccc ctgcccttcg
     6181 cctgggacat cctgtccccc cagttcatgt acggctccaa ggcgtacgtg aagcaccccg
     6241 ccgacatccc cgattacaag aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga
     6301 tgaacttcga ggacggcggt ctggtgaccg tgacccagga ctcctccctg caggacggca
     6361 cgctgatcta caaggtgaag atgcgcggca ccaacttccc ccccgacggc cccgtaatgc
     6421 agaagaagac catgggctgg gaggcctcca ccgagcgcct gtacccccgc gacggcgtgc
     6481 tgaagggcga gatccaccag gccctgaagc tgaaggacgg cggccactac ctggtggagt
     6541 tcaagaccat ctacatggcc aagaagcccg tgcaactgcc cggctactac tacgtggaca
     6601 ccaagctgga catcacctcc cacaacgagg actacaccat cgtggaacag tacgagcgct
     6661 ccgagggccg ccaccacctg ttcctgtacg gcatggacga gctgtacaag taagcggccg
     6721 cgactctaga tcataatcag ccata
//
