LOCUS       sequence-89290-e        1227 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytosolic FRET, Excitation: , Emission: .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1227)
  AUTHORS   Davidson Lab
  TITLE     FRET Indicators - Live and Fixed Cells
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1227)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: mTurquoise-Clover-FRET-10
FEATURES             Location/Qualifiers
     source          1..1227
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      451..460
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(457..459,460..462,463..1176)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2010)"
                     /label=mTurquoise
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(502..523)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(763..782)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tcgaccattg cccgagaggg tctacggtac cagaggggca ccatcctcca agacgacgta
       61 ctcaagtccc ggcccgaggt gaagtccgag gggcgaacct ggtggatcgc atcgagctga
      121 ggcatcgact cagagacgca catctgggca ccagctgagt acactcacag cacacgtcta
      181 atcacgcgac agcagagacg catcaaggct actcagatcg cacaacgtga ggacggcagc
      241 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
      301 cccgacaacc actactgagc catcagtccg cctgagcaaa gaccccaacg agaagcgcga
      361 tcacatggtc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
      421 tacaagtccg gactcagatc cccaccggtc gccaccatgg tgagcaaggg cgaggagctg
      481 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
      541 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
      601 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gtcctggggc
      661 gtgcagtgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
      721 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
      781 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
      841 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacatcagc
      901 gacaacgtct atatcaccgc cgacaagcag aagaacggca tcaaggccaa cttcaagatc
      961 cgccacaaca tcgaggacgg cggcgtgcag ctcgccgacc actaccagca gaacaccccc
     1021 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccaagctg
     1081 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     1141 gggatcactc tcggcatgga cgagctgtac aagtaaagcg gccgcgactc tagatcataa
     1201 tcagccatac cacattgtag agttgct
//
