LOCUS       sequence-89301-e        1278 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: N/A, Emission: N/A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1278)
  TITLE     Davidson plasmids
REFERENCE   2  (bases 1 to 1278)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: H2B-6
FEATURES             Location/Qualifiers
     source          1..1278
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             283..660
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      673..682
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             679..1224
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
ORIGIN
        1 ctgcccatag atatataagg cgcggtttga atctcaccgg gaattccaag gttcccaact
       61 catgaacgtc atggaagttt gtttggcacc caaaaatcaa tcgggagctt tccaaatgtc
      121 gtaacaactc cgcccccatt tgacgcaatg ggcggtaggc gtgtacggtg ggaggttctt
      181 atattaagca gagctggttt agtgaacgtc agatccgcta gcgctaccgg actcagatct
      241 cgagctcaag cttcgaattc tgcagtcgac ggtaccgcca ccatgccaga gccagcgaag
      301 tctgctcccg ccccgaaaaa gggctccaag aaggcggtga ctaaggcgca gaagaaaggc
      361 ggcaagaagc gcaagcgcag ccgcaaggag agctattcca tctatgtgta caaggttctg
      421 aagcaggtcc accctgacac cggcatttcg tccaaggcca tgggcatcat gaattcgttt
      481 gtgaacgaca ttttcgagcg catcgcaggt gaggcttccc gcctggcgca ttacaacaag
      541 cgctcgacca tcacctccag ggagatccag acggccgtgc gcctgctgct gcctggggag
      601 ttggccaagc acgccgtgtc cgagggtact aaggccatca ccaagtacac cagcgctaag
      661 gatccaccgg tcgccaccat ggacaaagac tgcgaaatga agcgcaccac cctggatagc
      721 cctctgggca agctggaact gtctgggtgc gaacagggcc tgcaccgtat catcttcctg
      781 ggcaaaggaa catctgccgc cgacgccgtg gaagtgcctg ccccagccgc cgtgctgggc
      841 ggaccagagc cactgatgca ggccaccgcc tggctcaacg cctactttca ccagcctgag
      901 gccatcgagg agttccctgt gccagccctg caccacccag tgttccagca ggagagcttt
      961 acccgccagg tgctgtggaa actgctgaaa gtggtgaagt tcggagaggt catcagctac
     1021 agccacctgg ccgccctggc cggcaatccc gccgccaccg ccgccgtgaa aaccgccctg
     1081 agcggaaatc ccgtgcccat tctgatcccc tgccaccggg tggtgcaggg cgacctggac
     1141 gtggggggct acgagggcgg gctcgccgtg aaagagtggc tgctggccca cgagggccac
     1201 agactgggca agcctgggct gggttaagcg gccgcgactc tagatcataa tcagccatac
     1261 cacattgtag agtttcct
//
