LOCUS       sequence-89304-e        1222 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nuclear Envelope, Excitation: N/A, Emission: N/A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1222)
  TITLE     Davidson plasmids
REFERENCE   2  (bases 1 to 1222)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: LaminA-C-18
FEATURES             Location/Qualifiers
     source          1..1222
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      40..49
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             46..591
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
ORIGIN
        1 cccatgctag tgacgtcaga tccgctagcg ctaccggtcg ccaccatgga caaagactgc
       61 gaaatgaagc gcaccaccct ggatagccct ctgggcaagc tggaactgtc tgggtgcgaa
      121 cagggcctgc accgtatcat cttcctgggc aaaggaacat ctgccgccga cgccgtggaa
      181 gtgcctgccc cagccgccgt gctgggcgga ccagagccac tgatgcaggc caccgcctgg
      241 ctcaacgcct actttcacca gcctgaggcc atcgaggagt tccctgtgcc agccctgcac
      301 cacccagtgt tccagcagga gagctttacc cgccaggtgc tgtggaaact gctgaaagtg
      361 gtgaagttcg gagaggtcat cagctacagc cacctggccg ccctggccgg caatcccgcc
      421 gccaccgccg ccgtgaaaac cgccctgagc ggaaatcccg tgcccattct gatcccctgc
      481 caccgggtgg tgcagggcga cctggacgtg gggggctacg agggcgggct cgccgtgaaa
      541 gagtggctgc tggcccacga gggccacaga ctgggcaagc ctgggctggg ttccggactc
      601 agatctggca gcggtggagg cagcgcatcc ggcggaagcg gaagcgagac cccgtcccag
      661 cggcgcgcca cccgcagcgg ggcgcaggcc agctccactc cgctgtcgcc cacccgcatc
      721 acccggctgc aggagaagga ggacctgcag gagctcaatg atcgcttggc ggtctacatc
      781 gaccgtgtgc gctcgctgga aacggagaac gcagggctgc gccttcgcat caccgagtct
      841 gaagaggtgg tcagccgcga ggtgtccggc atcaaggccg cctacgaggc cgagctcggg
      901 gatgcccgca agacccttga ctcagtagcc aaggagcgcg cccgcctgca gctggagctg
      961 agcaaagtgc gtgaggagtt taagagctga aagcgcgcat accaagaaga ggtgacctga
     1021 tagctgctca gctcggctga tgactgagct ctgctgactc caggagccgc actgagcact
     1081 gctctcatga gaagcgcacg ctgaggcgag ctgcatgatc tgcggggcag gtgcagctga
     1141 gcagcctagg tgaggccaga gcacttcagg atgaaatgct tccgccgatg aatgtctgaa
     1201 aacaggcttc gcgaaccatg ta
//
