LOCUS       sequence-8932-ex        1115 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Padua D, Zhang XH, Wang Q, Nadal C, Gerald WL, Gomis RR, Massague J
  TITLE     TGFbeta primes breast tumors for lung metastasis seeding through 
            angiopoietin-like 4.
  JOURNAL   Cell. 2008 Apr 4. 133(1):66-77.
  PUBMED    18394990
REFERENCE   2  (bases 1 to 1115)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2008 
            Apr 4. 133(1):66-77."
COMMENT     Sequence Label: pRetroSuper shSmad4J hygro
FEATURES             Location/Qualifiers
     source          1..1115
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(53..267)
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     complement(113..132)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     promoter        278..777
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(313..336)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     710..729
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
ORIGIN
        1 gggtgagttg gatgtatctc ttgaatacat tccaactgca caccggggat ctgtggtctc
       61 atacagaact tataagattc ccaaatccaa agacatttca cgtttatggt gatttcccag
      121 aacacatagc gacatgcaaa tattgcaggg cgccactccc ctgtccctca cagccatctt
      181 cctgccaggg cgcacgcgcg ctgggtgttc ccgcctagtg acactgggcc cgcgattcct
      241 tggagcgggt tgatgacgtc agcgttcgaa ttctaccggg taggggaggc gcttttccca
      301 aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta cacaagtggc
      361 ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc gttctttggt
      421 ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc cgccccgcag
      481 ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct cgtgcagatg
      541 gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca atagcagctt
      601 tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg cgggctcagg
      661 ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc attctgcacg
      721 cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg cctttcgacc
      781 tgcagcccaa gcttgccagc tggggcgccc tctggtaagg ttgggnaagc cctgcagatc
      841 tgatcccggg gggcaatgag atatgaaaaa gcctgaactc accgcgacgt ctgtcganaa
      901 attttctgac naanaaattc aaaggtccca ncaaannnnn tccctttnng gggnggnaaa
      961 aaaatncggn ntttcanctt nnaaaaaaag ggggggnaaa ttttnnnggg gnaaaaaaan
     1021 nnccnnaggg ttttnnnaaa aaaaattntt ttttttngng ntttttttnn ngggcccccc
     1081 ccnnaatttc cgaaanttnt taanatttgg naaat
//
