LOCUS       sequence-89379-e         834 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luciferase reporter plasmid for DE OCT4 enhancer activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Gafni O, Weinberger L, Mansour AA, Manor YS, Chomsky E, Ben-Yosef D,
            Kalma Y, Viukov S, Maza I, Zviran A, Rais Y, Shipony Z, Mukamel Z, 
            Krupalnik V, Zerbib M, Geula S, Caspi I, Schneir D, Shwartz T, Gilad
            S, Amann-Zalcenstein D, Benjamin S, Amit I, Tanay A, Massarwa R, 
            Novershtern N, Hanna JH
  TITLE     Derivation of novel human ground state naive pluripotent stem cells.
  JOURNAL   Nature. 2013 Dec 12;504(7479):282-6. doi: 10.1038/nature12745. Epub 
            2013 Oct 30.
  PUBMED    24172903
REFERENCE   2  (bases 1 to 834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature12745"; journalName: "Nature"; date: "2013-12-12- 
            12"; volume: "504"; issue: "7479"; pages: "282-6"
COMMENT     Sequence Label: pGL3-human OCT4 DE-SV40-Luc
FEATURES             Location/Qualifiers
     source          1..834
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      13..22
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(52..248)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      66..201
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 ttttggcgtc ttccatggtg gctttaccaa cagtaccgga atgccaagct ttttgcaaaa
       61 gcctaggcct ccaaaaaagc ctcctcacta cttctggaat agctcagagg ccgaggcggc
      121 ctcggcctct gcataaataa aaaaaattag tcagcgatgg ggcggagaat gggcggaact
      181 gggcggagtt aggggcggga tgggcggagt taggggcggg actatggttg ctgactaatt
      241 gagatgcaga tcgcagatct cgagctgagg ctcatgctgc tggtctagtg cttgattctg
      301 tttgcaagag aatagccaac ggaatgcctg tctgtgaggg atgatgtttg tctgtctgct
      361 cccaaaactt gatctcagtg gagggcctgg ggtaagtctg ggggctccag agggggctct
      421 gggccagggc tccccacagc ttcgaaggcc agaaggccag gtctggactg ggcacgctga
      481 cctctgtcga cttaagtaag gcttctcatt gcaggctcca ggctcagccc tgcctgggct
      541 tgtctgctgg ggtcagtggc tctgtctgcc ttctaagggg atgggtgtcc cgtggccagc
      601 tgtcttcatc ttggtggcat ccgtgagtct tttgagactt ttcccccact cttatgttgc
      661 ctctgttcgt gtgcccatct cctgtctgtg tagacttttt gagcctaatt gtatgcgtgc
      721 atttcaatac ctgccacagg tctgccggaa ggtctacaag gcagtggggt tggagctgtg
      781 ttcacttctc ggcctttaac tgcccaaaag gcaggtagat tatggggcct ggtg
//
