LOCUS       sequence-89395-e         776 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  BAC recombineering generated construct, used as a reporter for OCT4 
            expression .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Gafni O, Weinberger L, Mansour AA, Manor YS, Chomsky E, Ben-Yosef D,
            Kalma Y, Viukov S, Maza I, Zviran A, Rais Y, Shipony Z, Mukamel Z, 
            Krupalnik V, Zerbib M, Geula S, Caspi I, Schneir D, Shwartz T, Gilad
            S, Amann-Zalcenstein D, Benjamin S, Amit I, Tanay A, Massarwa R, 
            Novershtern N, Hanna JH
  TITLE     Derivation of novel human ground state naive pluripotent stem cells.
  JOURNAL   Nature. 2013 Dec 12;504(7479):282-6. doi: 10.1038/nature12745. Epub 
            2013 Oct 30.
  PUBMED    24172903
REFERENCE   2  (bases 1 to 776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature12745"; journalName: "Nature"; date: "2013-12-12- 
            12"; volume: "504"; issue: "7479"; pages: "282-6"
COMMENT     Sequence Label: delta DE OCT4-GFP-2A-PURO transgenic reporter
FEATURES             Location/Qualifiers
     source          1..776
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             130..729
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(130..149)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     626..646
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 aagtacagcg gtcgcgactc tagagtcgac ggttctggta agcaaacttt gaattttgac
       61 cttcttaagc ttgcgggaga cgtcgagtcc aaccccgggc ccgcatgcaa gcttcagctg
      121 aagcttacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc
      181 agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc
      241 gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc
      301 gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc
      361 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag
      421 ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg
      481 cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag
      541 ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc
      601 gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc
      661 gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc
      721 ggtgcctgag gcgcgagctt aagtttatct gcagaattcg cccttaaacg cgtaaa
//
