LOCUS       sequence-89455-e         907 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SNAP25B double mutant insensitive to both Botulinum toxin C1 & E; 
            N-terminal mCitrine tag connected by 4 amino acid linker. For 
            mammalian cells, used with mCer-4aa-VAMP2 to measure FRET.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Degtyar V, Hafez IM, Bray C, Zucker RS
  TITLE     Dance of the SNAREs: assembly and rearrangements detected with FRET 
            at neuronal synapses.
  JOURNAL   J Neurosci. 2013 Mar 27;33(13):5507-23. doi: 
            10.1523/JNEUROSCI.2337-12.2013.
  PUBMED    23536066
REFERENCE   2  (bases 1 to 907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.2337-12"; journalName: "J Neurosci"; date: 
            "2013-03-27- 27"; volume: "33"; issue: "13"; pages: "5507-23"
COMMENT     Sequence Label: mCit-Snap25b D179K A199R
FEATURES             Location/Qualifiers
     source          1..907
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    778..899
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(815..834)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     869..888
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 gacgagctgt acaagtccgg actcaccatg gccgaggacg cagacatgcg taatgaactg
       61 gaggagatgc agaggagggc tgaccagctg gctgatgagt ccctggaaag cacccgtcgc
      121 atgctgcagc tggtcgaaga gagtaaagat gctggcatca ggactttggt tatgttggat
      181 gagcaaggcg aacaactgga acgcattgag gaagggatgg accaaatcaa taaggatatg
      241 aaagaagcag aaaagaattt gacggaccta ggaaaattct gcgggctttg tgtgtgtccc
      301 tgtaacaagc ttaaatccag tgatgcttac aaaaaagcct ggggcaataa tcaggatgga
      361 gtagtggcca gccagcctgc ccgtgtggtg gatgaacggg agcagatggc catcagtggt
      421 ggcttcatcc gcagggtaac aaacgatgcc cgggaaaatg aaatggatga aaacctagag
      481 caggtgagcg gcatcatcgg aaacctccgt catatggccc tagacatggg caatgagatt
      541 gacacccaga atcgccagat taagaggatc atggagaagg ctgactccaa caaaaccaga
      601 attgatgaag ccaaccaacg tcggacaaag atgctgggaa gtggttaagg atccaccgga
      661 tctagataac tgatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
      721 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
      781 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
      841 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa
      901 ggcgtaa
//
