LOCUS       sequence-89465-e         754 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible expression of Cypridina luciferase and shRNAmir of 
            interest.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Charles JP, Fuchs J, Hefter M, Vischedyk JB, Kleint M, Vogiatzi F, 
            Schafer JA, Nist A, Timofeev O, Wanzel M, Stiewe T
  TITLE     Monitoring the dynamics of clonal tumour evolution in vivo using 
            secreted luciferases.
  JOURNAL   Nat Commun. 2014 Jun 3;5:3981. doi: 10.1038/ncomms4981.
  PUBMED    24889111
REFERENCE   2  (bases 1 to 754)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-06-3"; volume: "5:3981. doi"; pages: " 
            10.1038/ncomms4981"
COMMENT     Sequence Label: pIND-CLucZ
FEATURES             Location/Qualifiers
     source          1..754
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             134..736
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     633..653
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac atgtgtttag
       61 tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa
      121 acacgataat accatggcca ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga
      181 cgtcccccgg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca
      241 caccgtcgac ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac
      301 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt
      361 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gctcgcgcat
      421 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc
      481 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca
      541 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgctgg
      601 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg
      661 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg
      721 caagcccggt gcctgattaa ttaagcgcgt ctgg
//
