LOCUS       sequence-89479-e         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral expression of human pontin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Venteicher AS, Meng Z, Mason PJ, Veenstra TD, Artandi SE
  TITLE     Identification of ATPases pontin and reptin as telomerase components
            essential for holoenzyme assembly.
  JOURNAL   Cell. 2008 Mar 21;132(6):945-57. doi: 10.1016/j.cell.2008.01.019.
  PUBMED    18358808
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2008.01"; journalName: "Cell"; date: "2008-03-21- 
            21"; volume: "132"; issue: "6"; pages: "945-57"
COMMENT     Sequence Label: pMGIB-3xFLAG-pontin
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     336..352
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     promoter        382..400
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      461..470
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             482..547
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 cctcactcct tctctaggcg cccccatatg agatcttata tggggcaccc ccgccccttg
       61 taaacttccc tgaccctgac atgacaagag ttactaacag cccctctctc caagctcact
      121 tacaggctct ctacttagtc cagcacgaag tctggagacc tctggcggca gcctaccaag
      181 aacaactgga ccgaccggtg gtacctcacc cttaccgagt cggcgacaca gtgtgggtcc
      241 gccgacacca gactaagaac ctagaacctc gctggaaagg accttacaca gtcctgctga
      301 ccacccccac cgccctcaaa gtagacggca tcgcagcttg gatacacgcc gcccacgtaa
      361 aacaattgaa gatctagtcg aaattaaccc tcactaaagg gaacaaaagc tggagctcca
      421 ccgcggtggc ggccaattcg agctcggtac ccgtttaaac gccaccatgg ggtcccgggc
      481 tgactacaaa gaccatgacg gtgattataa agatcatgac atcgactaca aggatgacga
      541 tgacaagggg cgcgccaaga ttgaggaggt gaagagcact acgaagacgc agcgcatcgc
      601 ctcccacagc cacgtgaaag ggctggggct ggacgagagc ggcttggcca agcaggcggc
      661 ctcagggctt gtgggccagg agaacgcgcg agaggcatgt ggcgtcatag tagaattaat
      721 caaaagcaag aaaatggctg gaagagctgt cttgttggca ggacctcctg gaactggcaa
      781 gacagctctg gctctggcta ttgctcagga gctgggtagt aaggtcccct tctgcccat
//
