LOCUS       sequence-8948-ex        3462 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3462)
  AUTHORS   Zhang Y, Nash L, Fisher AL.
  TITLE     A simplified, robust, and streamlined procedure for the production 
            of C. elegans transgenes via recombineering
  JOURNAL   BMC Dev Biol. 2008 Dec 30;8(1):119.
  PUBMED    19116030
REFERENCE   2  (bases 1 to 3462)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Dev 
            Biol."; date: "2008-12-30"; volume: "8(1)"; pages: "119"
COMMENT     Sequence Label: pMOD4 galK-G
FEATURES             Location/Qualifiers
     source          1..3462
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             10..33
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        82..129
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     misc_feature    1452..1470
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase 
                     recognition (Reznikoff et al., 2004)"
     primer_bind     complement(1609..1628)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1728..1750
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1788..1806)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1874..1978
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1979..2047,2048..2839)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2197..2216)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     3297..3314
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3380..3398
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase 
                     recognition (Reznikoff et al., 2004)"
ORIGIN
        1 tctagaatgg attacaagga cgatgacgat aagatgagta aaggagaaga acttttcact
       61 ggagttgtcc caagaattcc tgttgacaat taatcatcgg catagtatat cggcatagta
      121 taatacgaca aggtgaggaa ctaaacccag gaggcagatc atgagtctga aagaaaaaac
      181 acaatctctg tttgccaacg catttggcta ccctgccact cacaccattc aggcgcctgg
      241 ccgcgtgaat ttgattggtg aacacaccga ctacaacgac ggtttcgttc tgccctgcgc
      301 gattgattat caaaccgtga tcagttgtgc accacgcgat gaccgtaaag ttcgcgtgat
      361 ggcagccgat tatgaaaatc agctcgacga gttttccctc gatgcgccca ttgtcgcaca
      421 tgaaaactat caatgggcta actacgttcg tggcgtggtg aaacatctgc aactgcgtaa
      481 caacagcttc ggcggcgtgg acatggtgat cagcggcaat gtgccgcagg gtgccgggtt
      541 aagttcttcc gcttcactgg aagtcgcggt cggaaccgta ttgcagcagc tttatcatct
      601 gccgctggac ggcgcacaaa tcgcgcttaa cggtcaggaa gcagaaaacc agtttgtagg
      661 ctgtaactgc gggatcatgg atcagctaat ttccgcgctc ggcaagaaag atcatgcctt
      721 gctgatcgat tgccgctcac tggggaccaa agcagtttcc atgcccaaag gtgtggctgt
      781 cgtcatcatc aacagtaact tcaaacgtac cctggttggc agcgaataca acacccgtcg
      841 tgaacagtgc gaaaccggtg cgcgtttctt ccagcagcca gccctgcgtg atgtcaccat
      901 tgaagagttc aacgctgttg cgcatgaact ggacccgatc gtggcaaaac gcgtgcgtca
      961 tatactgact gaaaacgccc gcaccgttga agctgccagc gcgctggagc aaggcgacct
     1021 gaaacgtatg ggcgagttga tggcggagtc tcatgcctct atgcgcgatg atttcgaaat
     1081 caccgtgccg caaattgaca ctctggtaga aatcgtcaaa gctgtgattg gcgacaaagg
     1141 tggcgtacgc atgaccggcg gcggatttgg cggctgtatc gtcgcgctga tcccggaaga
     1201 gctggtgcct gccgtacagc aagctgtcgc tgaacaatat gaagcaaaaa caggtattaa
     1261 agagactttt tacgtttgta aaccatcaca aggagcagga cagtgctgag gatcccttct
     1321 tgagtttgta acagctgctg ggattacaca tggcatggac gaactataca aaagcccaca
     1381 agctttgggt accgagctcg aattcgagcc aatatgcgag aacacccgag aaaattcatc
     1441 gatgatggtt gagatgtgta taagagacag ctgtcgtaat agcgaagagg cccgcaccga
     1501 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct
     1561 ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc
     1621 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg
     1681 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
     1741 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg
     1801 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     1861 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     1921 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     1981 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     2041 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     2101 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     2161 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     2221 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     2281 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     2341 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     2401 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     2461 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     2521 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     2581 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     2641 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     2701 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     2761 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     2821 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     2881 aaaacttcat ttttaatttg tgcatccgaa gatcagcagt tcaacctgtt gatagtacgt
     2941 actaagctct catgtttcac gtactaagct ctcatgttta acgtactaag ctctcatgtt
     3001 taacgaacta aaccctcatg gctaacgtac taagctctca tggctaacgt actaagctct
     3061 catggctaac gtactaagct ctcatgtttg aacaataaaa ttaatataaa tcagcaactt
     3121 aaatagcctc taaggtttta agttttataa gaaaaaaaag aatatataag gcttttaaag
     3181 ctagctttta aggtttcacc atgttctttc ctgcgttatc ccctgattct gtggataacc
     3241 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     3301 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     3361 ggccgattca ttaagacagc tgtctcttat acacatctca accctgaagc tcttgttggc
     3421 tagtgcgtag tcgttggcaa gcttgcatgc ctgcaggtcg ac
//
