LOCUS       sequence-89529-e         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of human 6His-tagged SUMO1 and SUMO-E1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Weber AR, Schuermann D, Schar P
  TITLE     Versatile Recombinant SUMOylation System for the Production of 
            SUMO-Modified Protein.
  JOURNAL   PLoS One. 2014 Jul 9;9(7):e102157. doi: 
            10.1371/journal.pone.0102157. eCollection 2014.
  PUBMED    25007328
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-07-9"; volume: "9(7):e102157. doi"; pages: " 
            10.1371/journal.pone.0102157. eCollection 2014"
COMMENT     Sequence Label: pSA1
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        110..128
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    129..153
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             209..226
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        658..676
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    677..701
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 attgactctc ttccgggcgc tatcatgcca taccgcgaaa ggttttgcgc cattcgatgg
       61 tgtccgggat ctcgacgctc tcccttatgc gactcctgca ttaggaaatt aatacgactc
      121 actatagggg aattgtgagc ggataacaat tcccctgtag aaataatttt gtttaacttt
      181 aataaggaga tataccatgg gcagcagcca tcaccatcat caccacagcc aggatctggt
      241 tccgcgtatg tctgaccagg aggccaaacc ttcaactgag gacttggggg ataagaagga
      301 aggtgaatat attaaactca aagtcattgg acaggatagc agtgagattc acttcaaagt
      361 gaaaatgaca acacatctca agaaactcaa agaatcatac tgtcaaagac agggtgttcc
      421 aatgaattca ctcaggtttc tctttgaggg tcagagaatt gctgataatc atactccaaa
      481 agaactggga atggaggaag aagatgtgat tgaagtttat caggaacaaa cggggggtta
      541 gggatccccg ggaattcatc gtgactgact gacgctgcag gtcgacaagc ttgcggccgc
      601 ataatgctta agtcgaacag aaagtaatcg tattgtacac ggccgcataa tcgaaattaa
      661 tacgactcac tataggggaa ttgtgagcgg ataacaattc cccatcttag tatattagtt
      721 aagtataaga aggagatata catatggcag atctcaattc ccgggtcgac atggcactgt
      781 cgcgggggct gccccgggag ctggctgagg cggtggccgg gggccgggtg ctggtggtg
//
