LOCUS       sequence-89603-e         943 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of human 6His-tagged SUMO3 and SUMO-E1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Weber AR, Schuermann D, Schar P
  TITLE     Versatile Recombinant SUMOylation System for the Production of 
            SUMO-Modified Protein.
  JOURNAL   PLoS One. 2014 Jul 9;9(7):e102157. doi: 
            10.1371/journal.pone.0102157. eCollection 2014.
  PUBMED    25007328
REFERENCE   2  (bases 1 to 943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-07-9"; volume: "9(7):e102157. doi"; pages: " 
            10.1371/journal.pone.0102157. eCollection 2014"
COMMENT     Sequence Label: pSA3
FEATURES             Location/Qualifiers
     source          1..943
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        117..135
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    136..160
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             216..233
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             255..530
                     /codon_start=1
                     /product="human small ubiquitin-related modifier 3"
                     /label=SUMO3
                     /translation="MSEEKPKEGVKTENDHINLKVAGQDGSVVQFKIKRHTPLSKLMKA
                     YCERQGLSMRQIRFRFDGQPINETDTPAQLEMEDEDTIDVFQQQTGG"
     promoter        647..665
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    666..690
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 accctgaatt gactctcttc cgggcgctat catgccatac cgcgaaaggt tttgcgccat
       61 tcgatggtgt ccgggatctc gacgctctcc cttatgcgac tcctgcatta ggaaattaat
      121 acgactcact ataggggaat tgtgagcgga taacaattcc cctgtagaaa taattttgtt
      181 taactttaat aaggagatat accatgggca gcagccatca ccatcatcac cacagccagg
      241 atctggttcc gcgtatgtcc gaggagaagc ccaaggaggg tgtgaagaca gagaatgacc
      301 acatcaacct gaaggtggca gggcaggacg gctccgtggt gcagttcaag atcaagaggc
      361 acacgccgct gagcaagctg atgaaggcct actgcgagag gcagggcttg tcaatgaggc
      421 agatcagatt caggttcgac gggcagccaa tcaatgaaac tgacactcca gcacagctgg
      481 agatggagga cgaggacacc atcgacgtgt tccagcagca gacgggaggt tagctcgagc
      541 ggccgcatcg tgactgactg acgctgcagg tcgacaagct tgcggccgca taatgcttaa
      601 gtcgaacaga aagtaatcgt attgtacacg gccgcataat cgaaattaat acgactcact
      661 ataggggaat tgtgagcgga taacaattcc ccatcttagt atattagtta agtataagaa
      721 ggagatatac atatggcaga tctcaattcc cgggtcgaca tggcactgtc gcgggggctg
      781 ccccgggagc tggctgaggc ggtggccggg ggccgggtgc tggtggtggg ggcgggcggc
      841 atcggctgcg agctcctcaa gaatctcgtg ctcaccggtt tctcccacat cgacctgatt
      901 gatctggata ctattgatgt aagcaacctc aacagacagt ttt
//
