LOCUS       sequence-8974-ex         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Sancak Y, Peterson TR, Shaul YD, Lindquist RA, Thoreen CC, Bar-Peled
            L, Sabatini DM
  TITLE     The Rag GTPases bind raptor and mediate amino acid signaling to 
            mTORC1.
  JOURNAL   Science. 2008 Jun 13. 320(5882):1496-501.
  PUBMED    18497260
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2008 Jun 13. 320(5882):1496-501."
COMMENT     Sequence Label: Flag pLJM1 Rheb1
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             71..94
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    734..851
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gtacggtggg aggtctatat aagcagagct ggtttagtga accgtcagat ccgctagcgc
       61 taccggtatg gactacaagg atgacgatga caagggtggt ggtggtggtg cgtcgacgat
      121 gccgcagtcc aagtcccgga agatcgcgat cctgggctac cggtctgtgg ggaaatcctc
      181 attgacgatt caatttgttg aaggccaatt tgtggactcc tacgatccaa ccatagaaaa
      241 cacttttaca aagttgatca cagtaaatgg acaagaatat catcttcaac ttgtagacac
      301 agccgggcaa gatgaatatt ctatctttcc tcagacatac tccatagata ttaatggcta
      361 tattcttgtg tattctgtta catcaatcaa aagttttgaa gtgattaaag ttatccatgg
      421 caaattgttg gatatggtgg ggaaagtaca aatacctatt atgttggttg ggaataagaa
      481 agacctgcat atggaaaggg tgatcagtta tgaagaaggg aaagctttgg cagaatcttg
      541 gaatgcagct tttttggaat cttctgctaa agaaaatcag actgctgtgg atgtttttcg
      601 aaggataatt ttggaggcag aaaaaatgga cggggcagct tcacaaggga agtcttcatg
      661 ctcggtgatg tgagattatc tgagatatct tcgaattctc gacctcgaga caaatggcag
      721 tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgcn ggggaaagaa
      781 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
      841 ttcaaaattt tcgggtttat tacnggacag caganatcca ctttggccgc ggctcgaggg
//
