LOCUS       sequence-89759-e         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Csy4 in Mammalian cells for site-specific RNA processing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Nissim L, Perli SD, Fridkin A, Perez-Pinera P, Lu TK
  TITLE     Multiplexed and Programmable Regulation of Gene Networks with an 
            Integrated RNA and CRISPR/Cas Toolkit in Human Cells.
  JOURNAL   Mol Cell. 2014 May 14. pii: S1097-2765(14)00355-4. doi: 
            10.1016/j.molcel.2014.04.022.
  PUBMED    24837679
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2014 May 14. pii: S1097-2765(14)00355-4. doi: 
            10.1016/j.molcel.2014.04.022."
COMMENT     Sequence Label: PGK1p-Csy4-pA (Construct 2)
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(720..740)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(741..758)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttgatcctag cagaagcaca ggctgcaggg tgacggtcca tcccgctctc ctgggcacaa
       61 gacatgggca gcgtgccatc atcctgcgcc gccaccgccg gcgggaagcc atggctgata
      121 tcttatgcgg ccgcttagag ctctcatcag aaccagggca cgaagccgcc tttagacagg
      181 ccatagcatg taaatccgcc ctcctcggct gtcacctgca ggggcccgtg tctgatgaac
      241 agtctgaaat gctggccagt gctctggctc cgcagggtca caaatggcag atccagggct
      301 ctggccactg tgtcggggat tctctttctg gcctcctcct cgctcagatc atgtcgcctc
      361 atcagcctcc gccgcagtct ctcgggattt gacttggcct gcacgcggct cacctggcgg
      421 tagggtgttg ggtgtggcac cacggcgggc tcgccaaact gcagatgatc ccgcaggccc
      481 tccagccaag gccgggccag cagggcgcgc agatcgtctg cgctggcgtg gattctcagc
      541 cgctcgccca ggcgactccg ggactcgtcc aggtcgggga aggacacgcc gattctgtct
      601 ccgccttggg cgaccagagc ctgatgcagc ttgccaaaca gcacgctcat cagctgggcg
      661 ggagggaact caggatcggg cctcagtctg atgtccagat agtggtcgct agctgcattg
      721 gattggaagt acaggttttc atggtgatgg tgatggtgag aagatttcat ggtggcacgc
      781 gtaagcttgg gatgcaggtc gaaaggcccg gagatgagga agaggagaac agcgcggcag
      841 acgtgcg
//
