LOCUS       sequence-89761-e        1061 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-regulated lentiviral shRNA vector. Cre addition causes EGFP to 
            be recombined out of the construct, activating shRNA expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Ventura A, Meissner A, Dillon CP, McManus M, Sharp PA, Van Parijs L,
            Jaenisch R, Jacks T
  TITLE     Cre-lox-regulated conditional RNA interference from transgenes.
  JOURNAL   Proc Natl Acad Sci U S A. 2004 Jul 13. 101(28):10380-5.
  PUBMED    15240889
REFERENCE   2  (bases 1 to 1061)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2004 Jul 13. 101(28):10380-5."
COMMENT     Sequence Label: pSico
FEATURES             Location/Qualifiers
     source          1..1061
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(77..280)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(106..126)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        281..584
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     protein_bind    684..717
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     primer_bind     complement(735..755)
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
ORIGIN
        1 ccggtgaaca gctcctcgcc cttgctcacc atggtggcga ccggtagcgc tagcggatct
       61 gacggttcac taaaccagct ctgcttatat agacctccca ccgtacacgc ctaccgccca
      121 tttgcgtcaa tggggcggag ttgttacgac attttggaaa gtcccgttga ttttggtgcc
      181 aaaacaaact cccattgacg tcaatggggt ggagacttgg aaatccccgt gagtcaaacc
      241 gctatccacg cccattgatg tactgccaaa accgcatcac catggtaata gcgatgacta
      301 atacgtagat gtactgccaa gtaggaaagt cccataaggt catgtactgg gcataatgcc
      361 aggcgggcca tttaccgtca ttgacgtcaa tagggggcgt acttggcata tgatacactt
      421 gatgtactgc caagtgggca gtttaccgta aatactccac ccattgacgt caatggaaag
      481 tccctattgg cgttactatg ggaacatacg tcattattga cgtcaatggg cgggggtcgt
      541 tgggcggtca gccaggcggg ccatttaccg taagttatgt aacgcggaac tccatatatg
      601 ggctatgaac taatgacccc gtaattgatt actattaata actaatgcat ggcggtaata
      661 cggaattcaa aaaaccaagg cttataactt cgtataattt atactatacg aagttataat
      721 tactttacag ttagggtgag tttccttttg tgctgttttt taaaataata atttagtatt
      781 tgtatctctt atagaaatcc aagcctatca tgtaaaatgt agctagtatt aaaaagaaca
      841 gattatctgt cttttatcgc acattaagcc tctatagtta ctaggaaata ttatatgcaa
      901 attaaccggg gcaggggagt agccgagctt ctcccacaag tctgtgcgag ggggccggcg
      961 cgggcctaga gatggcgcgt cggatctcta gagcgggccc ggtactaacc aaactggatc
     1021 tctgctgtcc ctgtaataaa cccgaaaatt ttgaattttt g
//
