LOCUS       sequence-89770-e         999 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Yu X, Zhan X, D'Costa J, Tanavde VM, Ye Z, Peng T, Malehorn MT, Yang
            X, Civin CI, Cheng L
  TITLE     Lentiviral vectors with two independent internal promoters transfer 
            high-level expression of multiple transgenes to human hematopoietic 
            stem-progenitor cells.
  JOURNAL   Mol Ther. 2003 Jun . 7(6):827-38.
  PUBMED    12788657
REFERENCE   2  (bases 1 to 999)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther. 
            2003 Jun . 7(6):827-38."
COMMENT     Sequence Label: EF.CMV.RFP
FEATURES             Location/Qualifiers
     source          1..999
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      155..164
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(246..449)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(275..295)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        450..753
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(853..872)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(854..872)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(921..941)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 gggccgccct tggtcacctt cagcttcacg gtgttgtggc cctcgtaggg gcggccctcg
       61 ccctcgccct cgatctcgaa ctcgtggccg ttcacggtgc cctccatgcg caccttgaag
      121 cgcatgaact cggtgatgac gttctcggag gaggccatgg tggcgaccgg tggatccccc
      181 aattcgaagc ttgagctcga gatctgagtc cggtagcgct agcggatctg acggttcact
      241 aaaccagctc tgcttatata gacctcccac cgtacacgcc taccgcccat ttgcgtcaat
      301 ggggcggagt tgttacgaca ttttggaaag tcccgttgat tttggtgcca aaacaaactc
      361 ccattgacgt caatggggtg gagacttgga aatccccgtg agtcaaaccg ctatccacgc
      421 ccattgatgt actgccaaaa ccgcatcacc atggtaatag cgatgactaa tacgtagatg
      481 tactgccaag taggaaagtc ccataaggtc atgtactggg cataatgcca ggcgggccat
      541 ttaccgtcat tgacgtcaat agggggcgta cttggcatat gatacacttg atgtactgcc
      601 aagtgggcag tttaccgtaa atactccacc cattgacgtc aatggaaagt ccctattggc
      661 gttactatgg gaacatacgt cattattgac gtcaatgggc gggggtcgtt gggcggtcag
      721 ccaggcgggc catttaccgt aagttatgta acgcggaact ccatatatgg gctatgaact
      781 aatgaccccg taattgatta ctattagggc tgcaggaatt cgatatcaag cttacctagc
      841 cagcttgggt ctccctatag tgagtcgtat tagtaccaag ctaattcctc acgacacctg
      901 aaatggaaga aaaaaacttt gaaccactgt ctgaggcttg agaatgaacc aagatccaaa
      961 ctcaaaaagg gcaaattcca aggagaatta catcaagtg
//
