LOCUS       sequence-89808-e        3999 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GeneGuard vector cassette: dapA, R6K COR, Zeta toxin; with ampR as 
            cargo..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3999)
  AUTHORS   Wright O, Delmans M, Stan GB, Ellis T
  TITLE     GeneGuard: a Modular Plasmid System Designed for Biosafety.
  JOURNAL   ACS Synth Biol. 2014 May 13.
  PUBMED    24847673
REFERENCE   2  (bases 1 to 3999)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 May 13."
COMMENT     Sequence Label: pSEVAD1Z1A
FEATURES             Location/Qualifiers
     source          1..3999
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(10..49)
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     CDS             complement(join(53..844,845..913))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(914..1005)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    1066..1122
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     terminator      1165..1259
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      3488..3876
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     terminator      complement(3907..3993)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaattaagt ccaaaaaaaa aggctccaaa aggagccttt aattgtatcg gtttaccaat
       61 gtttaatcag gctcgcgcca atttccgcaa tctgacggtt acgttcatcc atcgtcgcct
      121 ggctgccggt ggtataaatc accacaatac ggctcggttt accatccggg cccagcgccg
      181 caataatgcc acggctgcca cgttcgcccg caccgctttt atccgcaata aaccagccag
      241 ccggcagcgc gctacgcagc agcggacccg ccactttatc cgcttccatc caatcaatca
      301 gttgctggcg gcttgccagg gtcagcagct cacccgtcag cagtttacgc agggtggtcg
      361 ccattgctgc cggcatggtg gtatcacgtt catcgttcgg aatcgcttcg ttcagttccg
      421 gttcccaacg atccagacgg gtcacatgat cgcccatgtt atgcagaaac gcggtcagtt
      481 ctttcggacc gccaatggtc gtcagcagca ggttcgccgc ggtgttatcg ctcatggtaa
      541 tcgccgcgct gcacagttca cgcacggtca tgccatcggt cagatgtttt tcggtcaccg
      601 ggctatattc caccagatcg ttctggctat aatgaatacg acggcccagt tgttcctggc
      661 ccgcatccac acggctcaga accgcaccgc acagcagcac tttaaaggtg ctcatcatcg
      721 gaaaacgttc ttccggacga aaagattcca gaattttgcc gctgttcaga tccagttcaa
      781 tatagcccac gcgcgcaccc agttgatctt ccgcatcttt cactttcacc agggtttccg
      841 gatgcgcaaa caccggcagg caaaacgccg caaaaaacgg aatcagcgcc acacgaaaat
      901 gctgaatgct catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
      961 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gaattaaaaa
     1021 aaagcccgct cattaggcgg gctactacct aggccgcggc cgcgcgaatt cgagctcggt
     1081 acccggggat cctctagagt cgacctgcag gcatgcaagc ttgcggccgc gtcgtgactg
     1141 ggaaaaccct ggcgactagt cttggactcc tgttgataga tccagtaatg acctcagaac
     1201 tccatctgga tttgttcaga acgctcggtt gccgccgggc gttttttatt ggtgagaatc
     1261 caggggtccc caataattac gatttaaatc acagcaaaca ccacgtcggc cctatcagct
     1321 gcgtgctttc tatgagtcgt tgctgcataa cttgacaatt aacatccggc tcgtagggtt
     1381 tgtggagggc ccaagttcac ttaaaaagga gatcaacaat gaaagcaatt ttcgtactga
     1441 aacatcttaa tcatgctggg gagggtttct aatgttcacg ggaagtattg tcgcgattgt
     1501 tactccgatg gatgaaaaag gtaatgtctg tcgggctagc ttgaaaaaac tgattgatta
     1561 tcatgtcgcc agcggtactt cggcgatcgt ttctgttggc accactggcg agtccgctac
     1621 cttaaatcat gacgaacatg ctgatgtggt gatgatgacg ctggatctgg ctgatgggcg
     1681 cattccggta attgccggga ccggcgctaa cgctactgcg gaagccatta gcctgacgca
     1741 gcgcttcaat gacagtggta tcgtcggctg cctgacggta accccttact acaatcgtcc
     1801 gtcgcaagaa ggtttgtatc agcatttcaa agccatcgct gagcatactg acctgccgca
     1861 aattctgtat aatgtgccgt cccgtactgg ctgcgatctg ctcccggaaa cggtgggccg
     1921 tctggcgaaa gtaaaaaata ttatcggaat caaagaggca acagggaact taacgcgtgt
     1981 aaaccagatc aaagagctgg tttcagatga ttttgttctg ctgagcggcg atgatgcgag
     2041 cgcgctggac ttcatgcaat tgggcggtca tggggttatt tccgttacgg ctaacgtcgc
     2101 agcgcgtgat atggcccaga tgtgcaaact ggcagcagaa gggcattttg ccgaggcacg
     2161 cgttattaat cagcgtctga tgccattaca caacaaacta tttgtcgaac ccaatccaat
     2221 cccggtgaaa tgggcatgta aggaactggg tcttgtggcg accgatacgc tgcgcctgcc
     2281 aatgacacca atcaccgaca gtggccgtga gacggtcaga gcggcgctta aacatgccgg
     2341 tttgctgtaa gacttttgtc aggttcctac tgtgacgact accaccgata gactggagtg
     2401 ttgctgcgaa aaaaccccgc cgaagcgggg ttttttgcga gaagtcacca cgattgtgct
     2461 ttacacggag tagtcggcag ttccttaaat acctggaagt ttaggtgttt tgggtattgg
     2521 tggtgtaggt ggctgcaaac tttccaattt ttgttgaatt gctttaaatt caggtgtctc
     2581 ttggtgttta tttagaacca ttttttgctc tattcgttct aaagtcggtt gaatttcttt
     2641 ccctgatact ttacgattca attctttttc taaggtttct tttggactaa tagaaggtgt
     2701 ttccaaactt gaatagagtt ttactccttc tctgttatat agccttatat cgctaaataa
     2761 gcccgtttta tgaagggttt ctaaattggt cggtaagttt ttgacaacaa tatcatgcgc
     2821 ttgttttggt gttgccctgg ctgtcattgg atcatctgca tacatggttt catatcgttc
     2881 aattgttcct aaatatgagt tgattttagg tactgccatg acatacattt ttgtttcata
     2941 acctttggct tgaagcattg ttgcggtttg aataggaacg tctgttgttc gtcctgtacc
     3001 ttcgatcacc aaattatacc cttgatcgct caaacggctt atgatcgctt ctgtcatgcg
     3061 attagaataa ggggtaacgt gttttactac gtctttttca taaagtttca caagttcatc
     3121 aaaattaggg tgctgttgtt taaaggtatc attatcaatg acaataacat tcccttgtgt
     3181 ttcttcaaaa attgctgatc gcaaactggt tttccctgac cctggttgcc caccaagtaa
     3241 aaaagcggtt ggcgattcaa ccgctttttt tccttgaatc aattcttcta aattatcatt
     3301 taagcgattc tcaaattgtt tgtcagtaaa attgactata tttgccatct agtattgccc
     3361 gagtatctct agtaacattt attgtacaac acgagcccat ttttgtcaaa taaattttaa
     3421 attatatcaa cgttaataag acgttgtcaa taaaattatt ttgacaaaat tggccggccg
     3481 gcgcgccgat ctgaagatca gcagttcaac ctgttgatag tacgtactaa gctctcatgt
     3541 ttcacgtact aagctctcat gtttaacgta ctaagctctc atgtttaacg aactaaaccc
     3601 tcatggctaa cgtactaagc tctcatggct aacgtactaa gctctcatgt ttcacgtact
     3661 aagctctcat gtttgaacaa taaaattaat ataaatcagc aacttaaata gcctctaagg
     3721 ttttaagttt tataagaaaa aaaagaatat ataaggcttt taaagccttt aaggtttaac
     3781 ggttgtggac aacaagccag ggatgtaacg cactgagaag cccttagagc ctctcaaagc
     3841 aattttgagt gacacaggaa cacttaacgg ctgacatggg gcgcgcccag ctgtctaggg
     3901 cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa aacgaaaggc
     3961 ccagtctttc gactgagcct ttcgttttat ttgatgcct
//
