LOCUS       sequence-89875-e         939 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 939)
  TITLE     SGC_OxfordQ3Q4_2013
REFERENCE   2  (bases 1 to 939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CDC42BPAA-c004
FEATURES             Location/Qualifiers
     source          1..939
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(31..48)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(98..112)
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     CDS             complement(122..139)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(140..160)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 ctttcgggct ttgttagcag ccggatctca gtggtggtgg tggtggtgct cgagtgcggc
       61 cgcaagcttg tcgacggagc tcgaattcgg atcctcactt atcgtcgtca tccttgtatc
      121 aatggtggtg atgatggtgc gattggaagt agaggttctc tgctgaggtg ggacctgctg
      181 tgactcttaa acagctccga tcagaaagaa cacagctact agtatatgta aacccaacaa
      241 atggtagatg atggccagaa aatgcagtat gtgttggtgg gggcattgtt tcagagtttt
      301 ttaaacagtc gtcatccaca tcgaaatttg acgtgtctgt tggactacta acttctggaa
      361 tgtaaggtgc ttcacagttt cggatgttat cccaatcaat tccactgaaa aacggatgtt
      421 tcttaaagtc ttctattcca ttctggccaa gtcgatgttc tctgctacaa atgagtcttc
      481 gaataagatc tttagcattt tcagacacat cagtcacttg ggttggaaac tgaaacctct
      541 ctttatgatt cataattttt ccatatgtct ccaccagaga ttccgcatag aatggtgttt
      601 ctccgtaaag catctcatac atgcagactc ccaaggacca ccagtcacac tctggtccat
      661 atctgccttt cccgccctcc atagcctgaa ggatttccgg ggaaatgtag tctggagttc
      721 caactgccac tgaggactgg accgttccat cttccatcag cttcagacaa gaaccaaaat
      781 ctgctaaacg aatatgtcca ttcatatcca tcaatatatt gtcaggtttg atgtctctgt
      841 gaacatagtg gagctggtga actgagtcaa tggctatcac catctcagcc aagtaaaacc
      901 gagccatctc ttctggcaat cgatcttcaa atttgctga
//
