LOCUS       sequence-89912-e         779 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Gradia SD, Ishida JP, Tsai MS, Jeans C, Tainer JA, Fuss JO
  TITLE     MacroBac: New Technologies for Robust and Efficient Large-Scale 
            Production of Recombinant Multiprotein Complexes.
  JOURNAL   Methods Enzymol. 2017;592:1-26. doi: 10.1016/bs.mie.2017.03.008. 
            Epub 2017 May 15.
  PUBMED    28668116
REFERENCE   2  (bases 1 to 779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2017"; volume: "592:1-26. doi"; pages: " 
            10.1016/bs.mie.2017.03.008. Epub 2017 May 15"
COMMENT     Sequence Label: pFastBac His6 TEV cloning vector with BioBrick...
FEATURES             Location/Qualifiers
     source          1..779
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(184..189,190..206,207..212))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     primer_bind     621..638
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             758..775
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ctgctccata acatcaaaca tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga
       61 ggcatagact gtacaaaaaa acagtcataa caagccatga aaaccgccac tgcgccgtta
      121 ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg agcgcatacg ctacttgcat
      181 tacagtttac gaaccgaaca ggcttatgtc aactgggttc gtgccttcat ccgtttccac
      241 ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg aggcatttct gtcctggctg
      301 gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg cattggcggc cttgctgttc
      361 ttctacggca aggtgctgtg cacggatctg ccctggcttc aggagatcgg aagacctcgg
      421 ccgtcgcggc gcttgccggt ggtgctgacc ccggatgaag tggttcgcat cctcggtttt
      481 ctggaaggcg agcatcgttt gttcgcccag gactctagct atagttctag tggttggcta
      541 cgtatactcc ggactattaa tagatcatgg agataattag cggccgcgtt taaacatcca
      601 ccatttgatg gagataatta aaatgataac catctcgcaa ataaataagt attttactgt
      661 tttcgtaaca gttttgtaat aaaaaaacct ataactattc cggattattc ataccgtccc
      721 accatcgggc gcggatcccg gtccgatggg ttcttctcac catcaccatc accatgaaa
//
