LOCUS       sequence-89973-e         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Csy4 in Mammalian cells for site-specific RNA processing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Nissim L, Perli SD, Fridkin A, Perez-Pinera P, Lu TK
  TITLE     Multiplexed and Programmable Regulation of Gene Networks with an 
            Integrated RNA and CRISPR/Cas Toolkit in Human Cells.
  JOURNAL   Mol Cell. 2014 May 14. pii: S1097-2765(14)00355-4. doi: 
            10.1016/j.molcel.2014.04.022.
  PUBMED    24837679
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2014 May 14. pii: S1097-2765(14)00355-4. doi: 
            10.1016/j.molcel.2014.04.022."
COMMENT     Sequence Label: PGK1p-Csy4-pA (Construct 2)
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        239..738
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(274..297)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     671..690
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             780..797
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             798..818
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
       61 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttgcca ttcgccattc
      121 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagccc
      181 aagctaccat gataagtaag taatattaag gtacgggagg tacttggagg tacctaccgg
      241 gtaggggagg cgcttttccc aaggcagtct ggagcatgcg ctttagcagc cccgctgggc
      301 acttggcgct acacaagtgg cctctggcct cgcacacatt ccacatccac cggtaggcgc
      361 caaccggctc cgttctttgg tggccccttc gcgccacctt ctactcctcc cctagtcagg
      421 aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt
      481 ctcactagtc tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt aggcctttgg
      541 ggcagcggcc aatagcagct ttgctccttc gctttctggg ctcagaggct gggaaggggt
      601 gggtccgggg gcgggctcag gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc
      661 ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc
      721 tcatctccgg gcctttcgac ctgcatccca agcttacgcg tgccaccatg aaatcttctc
      781 accatcacca tcaccatgaa aacctgtact tccaatccaa tgcagctagc gaccactatc
      841 tggacatcag actgaggccc gatcctgagt
//
